潘生丁预处理对大鼠肝缺血/再灌注损伤的保护作用  

Dipyridamole preconditioning protects against ischemia/reperfusion injury of rat liver

作　　者：洪勇[1] 廖文胜[1] 莫瑞祥[1] 

机构地区：[1]桂林医学院附属南溪山医院肝胆外科,广西541002

出　　处：《中国危重病急救医学》2006年第7期425-427,F0005,共4页Chinese Critical Care Medicine

摘　　要：目的探讨潘生丁预处理对肝缺血/再灌注损伤的保护作用。方法SD大鼠30只,随机分为假手术组、缺血/再灌注组及潘生丁组,每组10只。常温下制备大鼠肝缺血/再灌注损伤模型,潘生丁组于缺血前30min经门静脉给予潘生丁10mg/kg加生理盐水至0.5ml,假手术组和缺血/再灌注组注入等量生理盐水,用小号无损伤钳阻断肝门45min后恢复血流灌注,并于1h后取门静脉血测定血清丙氨酸转氨酶(ALT)、乳酸脱氢酶(LDH)、肿瘤坏死因子α(TNFα)及内皮素1(ET1),同时取肝组织行病理组织学检查及腺苷酸含量测定。结果缺血/再灌注组血清ALT、LDH、TNFα及ET1均明显高于假手术组,潘生丁组则明显低于缺血/再灌注组(P均<0.01)。缺血/再灌注组肝组织中腺苷酸含量明显低于假手术组,潘生丁组则明显高于缺血/再灌注组(P均<0.01)。潘生丁组肝组织病理组织学改变明显轻于缺血/再灌注组,并接近假手术组。结论潘生丁预处理对肝缺血/再灌注损伤具有保护作用。Objective To investigate the protective mechanism of dipyridamole preconditioning against hepatic ischemia/reperfusion injury. Methods Thirty SD rats were randomly, divided into normal control group, ischemia/reperfusion group, and dipyridamole group, with 10 rats in each group. Using 45-minute ischemia/reperfusion rat model at normal temperature, 10 mg/kg of dipyridamole＋normal saline were injected into portal vein before ischemia. Only normal saline was injected in the ischemia/reperfusion group. An hour later, blood was obtained from the portal vein to determine the enzyme levels, including alanine aminotransferase （ALT） , lactate dehydrogenase （LDH） and tumor necrosis factor-α （TNF-α）, and endothelin-1（ET- 1）. The alteration in pathological morphology of the ischemia lobe was observed. The content of adenosine phosphates in the liver was determined. Results The ALT and LDH activity, TNF-α, ET-1 levels were significantly higher in the ischemia/reperfusion group than those in the control group （all P〈0.01）. The levels of these variables were much lower in the dipyridamole group than those of the ischemia/reperfusion group （P〈0. 01）, but little higher than those of the control group （P〉0.05）. The adenosine phosphates levels of ischemia/reperfusion group were significantly lower than those of the control group （P〈0.01）. They were much higher in the dipyridamole group than those of the ischemia/reperfusion group （P〈0. 01）, but little higher than those of the control group （P〉0.05）. The control group had obvious alteration in pathological morphology, but only slight alteration was found in dipyridamole group, compared with control group. Conclusion Dipyridamole preconditioning protects against ischemia/reperfusion injury of the liver.

关 键 词：潘生丁 缺血/再灌注损伤 肝 腺苷酸 

分 类 号：R363[医药卫生—病理学]