Inhibition of the α-mannosidase Man2c1 gene expression enhances adhesion of Jurkat cells  

作　　者：Li Qu Ji Yu Ju Shuang Ling Chen Yah Shi Zhi Guang Xiang Yi Qun Zhou Yun Tian Yin Liu Li Ping Zhu 

机构地区：[1]Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China

出　　处：《Cell Research》2006年第7期622-631,共10页细胞研究（英文版）

基　　金：This work was supported by the National Basic Research Program of China （No. 2001CB510004） ;by the National Natural Science foundation of China （No. 31070868）.

摘　　要：Protein N-glycosylation plays very important roles in immunity and α-mannosidase is one of the key enzymes in Nglycosylation. This paper reports that inhibition of α-mannosidase Man2c1 gene expression enhances adhesion of Jurkat T cells. In comparison to the controls with normal expression of the enzyme, Jurkat cells with the inhibition of Man2c1 gene expression （AS cell） formed larger aggregates in culture, indicating an enhancement of adhesion between the cells. mRNA differential display analysis discovered up-regulation of several adhesion molecule genes in the AS cell. Because of the pivotal role played by CD54-LFA-1 interaction in immune cell interaction, this study focused on the contribution of enhanced expression of CD54 and LFA-1 to the enhanced adhesion of AS Jurkat cells. These facts, including increased binding of AS cells to ICAM-1-Fc, Mg^2＋ activation of the binding of AS cells to ICAM-1-Fc and enhanced aggregation of AS cells, together with the inhibiting effect of a blocking CD1 la mAb on the binding to ICAM-1-Fc and aggregation of the cells demonstrate an important contribution of enhanced CD54-LFA-1 interaction to increased adhesion between AS cells. The enhanced CD54-LFA-1 interaction also resulted in increased adhesion between AS Jurkat T cells and Raji B cells. In addition, AS cells showed cytoskeletal rearrangement. The data imply a biological significance of MAN2C1 in T-cell functioning.

关 键 词：Man2c1 Jurkat T cell ADHESION CD54-LFA-1 CYTOSKELETON 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]