In vitro reassembly of nuclear envelopes and organelles in Xenopus egg extracts  

作　　者：Ping Lu Hui Zheng Zhonghe Zhai 

机构地区：[1]College of Life Sciences [2]Institute of Molecular Medicine, Peking University, Beijing 100871, China

出　　处：《Cell Research》2006年第7期632-640,共9页细胞研究（英文版）

摘　　要：We reconstituted bilayer nuclear membranes, multilayer membranes, and organelles from mixtures ofXenopus laevis egg extracts and demembranatedXenopus sperm nuclei. Varying proportions of the cytosolic and vesicular fractions from the eggs were used in the reconstitution mixtures. A cytosol：vesicle ratio of 10：1 promoted reassembly of the normal bilayer nuclear membrane with inserted nuclear pore complexes around the decondensed Xenopus sperm chromatin. A cytosol： vesicle ratio of 5：1 caused decondensed and dispersed sperm chromatin to be either surrounded by or divided by unusual multilayer membrane structures with inlaid pore complexes. A cytosol：vesicle ratio of 2.5：1 promoted reconstitution of mitochondria, endoplasmic reticulum networks, and Golgi apparatus. During reassembly of the endoplasmic reticulum and Golgi apparatus, vesicular fragments of the corresponding organelles fused together and changed their shape to form flattened cistemae, which were then stacked one on top of another.

关 键 词：cell-free system endoplasmic reticulum Golgi apparatus MITOCHONDRIA nuclear reconstitution Xenopus egg extracts 

分 类 号：Q24[生物学—细胞生物学]