L-精氨酸诱导人肝癌细胞凋亡的作用  

Inducing effects of L-arginine on human HCC cells

作　　者：张剑[1] 陈规划[1] 陆敏强[1] 杨扬[1] 蔡常洁[1] 许赤[1] 李华[1] 

机构地区：[1]中山大学附属第三医院肝脏移植中心,广州市510630

出　　处：《中华肝胆外科杂志》2006年第6期397-399,共3页Chinese Journal of Hepatobiliary Surgery

基　　金：国家重点基础研究发展计划（973计划2003CB515507）

摘　　要：目的探讨 L-精氨酸诱导人肝癌细胞凋亡的作用及机制。方法采用倒置显微镜、透射电镜和流式细胞仪,观察和分析不同浓度 L-精氨酸作用下体外培养人肝癌细胞株 QGY-7703的增殖和凋亡情况。结果 (1)低浓度 L-精氨酸对肝癌细胞生长和细胞大体形态无明显影响。高浓度的L-精氨酸引起肝癌细胞形态变圆、体积变小,细胞皱缩,细胞核固缩,核碎裂,染色质边聚,凋亡小体形成。(2)低浓度 L-精氨酸对肝癌细胞生长周期和增殖指数无影响;而高浓度的 L-精氨酸引起 G_0/G_1期较对照组显著升高,S 期显著降低,细胞增殖指数显著下降(P<0.05),而 G_2/M 无明显变化。(3)低浓度 L-精氨酸(1mmol/L)和对照组细胞未见凋亡峰(亚 G_1峰),凋亡率分别为2.1%和2.5%;而16mmol/L和64mmol/L 浓度组则出现典型的凋亡峰,凋亡率分别为12.4%和30.8%,较对照组显著增高(P<0.05)。结论高浓度的 L-精氨酸通过合成 NO,诱导肝癌细胞凋亡。Objective To investigate the inducing effects of L-arginine on apoptosis of human HCC cells and explore its mechanism. Methods Human HCC QGY-7730 cells were treated with different concentrations of L-arginine. The morphological changes in the cells were observed with invert microscopy and transmission electron microscopy. Flow cytometry was used to examine the cell cycle and apoptosis of the HCC cells. Results 1） After exposure to L-arginine at high concentration, cell shrinkage, chromatin condensation and margination, nuclear fragmentation and formation of apoptotic bodies were observed in the HCC cells. 2） L-arginine increased percentage of Ga phase cells decreased that of S phase cells at higher concentrations. It mainly acted on S phase cells and arrested cell growth cycle at the Ga phase. 3） L-arginine at the concentrations of 16 mmol/L and 64 mmol/L significantly induced apoptosis of HCC cells, increased apoptotic rate and proliferating index （P〈0. 05）. The typical sub-diploid peak before Ga phase was observed in the cells by flow cytometry. Conclusions At high concentrations, L-arginine can induce apoptosis of human HCC cells by producing nitric oxide.

关 键 词：癌 肝细胞 L-精氨酸 凋亡 

分 类 号：R735.7[医药卫生—肿瘤] R977.4[医药卫生—临床医学]