肝癌患者组织中凝血及纤溶因子的基因表达检测与分析  

Detection and analyses of coagulation and fibrolysis factor mRNA levels in tissue of hepatocellular carcinoma patients

作　　者：周奇[1] 李绍强[2] 粱力建[2] 彭宝岗[2] 甄宇洋[1] 苏才坤[1] 

机构地区：[1]中山大学第一附属医院黄埔院区外一科,广东广州510700 [2]中山大学第一附属医院肝胆外科,广东广州510080

出　　处：《中国病理生理杂志》2006年第7期1348-1352,共5页Chinese Journal of Pathophysiology

基　　金：广东省医学科研立项课题(No.B2006035)

摘　　要：目的:检测组织因子(TF)、尿激酶型纤溶酶原激活物(uPA)及其受体(uPAR)mRNA在肝细胞癌组织中的表达并探讨其临床意义。方法:利用RT-PCR法分别检测27例肝细胞癌、癌旁及27例正常肝组织中TF、uPA、uPAmRNA表达阳性率及相对表达强度,并结合临床病理资料进行分析。结果:TF、uPA、uPAR在肝细胞癌组织中阳性率及相对表达强度分别为62.96%(17/27)、70.37%(19/27)、77.78%(21/27);0.567±0.268、0.964±0.458、0.784±0.322,均显著高于癌旁组织及正常组织,差异显著(P<0.05)。3者在肝癌组织中相对表达强度与肿瘤大小及部分侵袭转移指标有关,其中TFmRNA表达强度在肝内及肝外转移及门脉癌栓组高于无肝内及肝外转移及无门脉癌栓组(P<0.05),uPAmRNA在有包膜侵润、肝内转移及门脉癌栓组高于无包膜侵润、无肝内转移及门脉癌栓组(P<0.05);uPARmRNA在有肝内转移及门脉癌栓组高于无肝内转移及门脉癌栓组(P<0.05)。经Pearson检验肝细胞癌患者TF、uPA和uPARmRNA表达呈正相关[TF-uPA:r=0.373(P<0.01),TF-uPAR:r=0.534(P<0.01),uPA-uPAR:r=0.365(P<0.01)]。结论:肝细胞癌组织中TF、uPA及uPAR的mRNA显著升高并与部分侵袭转移指标有关,提示3者可能在肝细胞癌的发生及侵袭转移起协同作用。AIM： To detect the mRNA expression of tissue factor （TF）, urokinase - type plasminogen activator （ u - PA） and urokinase - type plasminogen activator receptor （ u - PAR） in the samples of hepatocellular carcinoma （HCC） and adjacent tissue, and to elucidate their association with clinical significance. METHODS： The mRNA levels of TF, u - PA and u - PAR in 27 human HCC tissue samples with their adjacent tissue samples and 27 normal liver tissues were detected by RT - PCR. The relationship between the mRNA expression and their clinic - pathological data were also analyzed. RESULTS ： The expressive rate of TF, uPA and uPAR in HCC tissue samples was 62. 96% （ 17/27）, 70. 37% （ 19/27）, 77.78% （21/27）, respectively and relative expression intensity of TF, uPA and uPAR were 0. 567 ± 0. 268, 0. 964 ± 0. 458 and 0. 784 ± 0. 322, respectively, which were significantly higher than those in adjacent tissue group and normal liver group （ P 〈 0. 05）. TF, uPA and uPAR mRNA levels were all significantly higher in larger size and in the invasive and metastatic subgroup （ P 〈 0.05 ）. There had positive relationship between the mRNA expressions of these three factors. CONCLUSION ： The results indicate that TF, uPA and uPAR might play an important role in hepatic carcinogenesis, invasiveness and metastasis.

关 键 词：肝肿瘤 凝血致活酶 尿纤溶酶原激活物 基因表达 

分 类 号：R363[医药卫生—病理学]