易卒中型肾血管性高血压大鼠脑大动脉重塑类型  

The Types and the Mechanisms of Cerebrovascular Remodeling in Stroke-prone Renalvascular Hypertensive Rats

作　　者：崔春梅[1] 武庆庆[2] 温红梅[3] 林健雯[1] 王立志[1] 曾进胜[1] 

机构地区：[1]中山大学附属第一医院神经科脑血管专科 [2]中山大学附属第一医院肾脏内科 [3]中山大学附属第三医院康复科,广东省广州市510080

出　　处：《中国动脉硬化杂志》2006年第5期374-376,共3页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金(39940012;30271485);美国CMB基金(00730);卫生部临床学科重点项目(2004);广东省自然科学基金(990065;21906);广东省医药卫生科研基金(B1999035);广东省博士基金(984225);广东省自然科学基金团队项目(2000年);广东省自然科学基金攻关项目(B30303;2003D30301)

摘　　要：目的观察易卒中型肾血管性高血压大鼠脑动脉的结构变化。方法将易卒中型肾血管性高血压大鼠第4周和8周时处死,脑大动脉切片用HE染色后,测量这些大动脉重塑参数,对免疫组织化学对纤维连接素蛋白表达进行图象分析。结果易卒中型肾血管性高血压大鼠组血压高于对照组。易卒中型肾血管性高血压大鼠第4周时颈总动脉平滑肌细胞总数(282.5±59.6个)和纤维连接素蛋白表达量(184.3±2.4)较对照组(344.7±50.9个和181.4±3.2)减少,单个细胞面积增加;第8周时,管壁厚度(74.8±12.3μm)、横截面积(233428.3±76487.9μm2)、壁腔比(0.086±0.007)、壁面积比(0.00030±0.00011)、单个细胞面积(547.9±111.8μm2)和纤维连接素蛋白表达量(180.8±2.3)均增加,单位面积细胞数(282.5±59.6个)减少。易卒中型肾血管性高血压大鼠基底动脉横截面积第4周和第8周时(15867.4±1316.9μm2和22556.5±6485.6μm2)均高于对照组(13598.9±1090.8μm2),纤维连接素蛋白表达量第8周时(166.2±5.1)增加。第4周时易卒中型肾血管性高血压大鼠大脑中动脉管腔内直径(208.6±59.6μm)增加,壁腔比(0.094±0.048)和壁面积比(0.00150±0.00040)减少;第8周时管壁厚度(23.3±7.2μm2)、横截面积(16236.2±6538.4μm2)和平滑肌细胞总数(52.2±16.1个)增加,管腔内直径和壁面积比与对照组的差异仍有统计学意义。Aim To investigate the difference in the types and mechanisms of vascular remodeling among the different brain arteries. Methods The parameters of vascular remodeling and the smooth muscle cells （ SMC ） were measured through the HE staining photos of the arteries. Protein expression of fibronectin （FN） was determined by immunohistochemistry. Results The amount of SMC and FN expression level in carotid arteries decreased significantly while the SMC average area increased compared with SD at 4 weeks post-operation. At the end of eighth week., there was a significant increase in wall thickhess, wall area, wall-to-lumen diameter ratio, wall-to-area ratio, SMC average area and FN expression level and a decrease in SMC amount d specific area of RHRSP carotid arteries. Wall area in RHRSP basial arteries had been increased since the fourth week post-operation and FN expression increased at the eighth week. There was a significant differece in lumen diamerer, wall- to-lumen diameter ratio and wall-to-area ratio between the middle cerebral arteries of SD and RHRSP （4 or 8 weeks post-operation）. The wall thickness, wall area and the SMC amount of RHR middle cerebral arteries were significantly higher than SD at 8 weeks post-operation. Conclusions The difference in the types and mechanisms of vascular remodding in RHRSP brain arteries may be connected with different stroke behavior among them.

关 键 词：神经病学 肾血管性高血压 易卒中型 血管重塑 纤维连接素 血管平滑肌细胞 动物模型 大鼠 大脑中动脉 基底动脉 颈总动脉 

分 类 号：R74[医药卫生—神经病学与精神病学]