葡萄糖-6-磷酸酶多克隆抗体对大鼠血管源性脑水肿的治疗作用  

Therapeutic effect of glucose-6-phosphate polyclonal antibody on vasogenic brain edema in rats

作　　者：陶珍[1] 陆兵勋[1] 张运周 程岗[1] 

机构地区：[1]南方医科大学南方医院神经内科,广东广州510515

出　　处：《南方医科大学学报》2006年第7期1041-1043,共3页Journal of Southern Medical University

摘　　要：目的观察葡萄糖-6-磷酸酶多克隆抗体(G-6-PpAb)对大鼠血管源性脑水肿(VBE)的治疗作用。方法将60只Wistar大鼠随机分为正常、脑水肿、脑水肿甘露醇、脑水肿G-6-PpAb共4组。Wistar大鼠腹腔注射苯肾上腺素造成VBE模型,然后分别股静脉注射甘露醇、腹腔注射G-6-PpAb,用Evansblue(EB)法测定血脑屏障(BBB)的通透性,并以远红外水分分析仪分别测定各组脑灰、白质水分含量百分比。结果G-6-PpAb组同甘露醇组相比对降低BBB通透性及大脑白质水分含量均有显著效果(P<0.01),对灰质的脱水作用无显著性差异(P>0.05)。结论G-6-P活性与VBE中BBB的通透性改变有关,G-6-PpAb对VBE尤其是脑白质水肿有选择性治疗作用。Objective To observe the therapeutic effect ofglucose-6-phosphate polyclonal antibody （G-6-P pAb） on vasogenic brain edema （VBE） in rats. Methods Sixty Wistar rats were randomly divided into normal control group,. VBE group, mannitol-treated edema group, and G-6-P pAb-treated edema group. After establishment of rat models of VBE by intraperitoneal injection ofphenylephrine in the latter 3 groups, mannitol was injected through the femoral vein in mannitol group and G-6-P pAb injected intraperitoneally in G-6-P pAb group. The permeability of the blood-brain barrier （BBB） was determined by Evans blue （EB） extravasation method, and the brain water content in the gray and white matter measured with a moisture analyzer. Results G-6-P pAb administration significantly reduced the permeability of BBB as well as the water content in the white matter in comparison with mannitol treatment （P〈0.01）, but the two treatments showed no obvious difference in reducing the water content in the gray matter （P〉0.05）. Conclusion Changes in G-6-P activity results in BBB permeability alteration in the condition of VBE, and G-6-P pAb has a selective therapeutic effect against VBE, especially white matter edema.

关 键 词：葡萄糖-6-磷酸酶多克隆抗体 血管源性脑水肿 血脑屏障 酶屏障 

分 类 号：R741[医药卫生—神经病学与精神病学]