MMP-2、TIMP-2和VEGF在肾细胞癌中的表达及意义  

Expression of MMP-2,TIMP-2 and VEGF in Renal Cell Carcinoma

作　　者：吴小燕[1] 张波[1] 徐联芳[1] 曾俊[1] 田斌群[1] 

机构地区：[1]武汉大学中南医院内科,武汉430071

出　　处：《武汉大学学报（医学版）》2006年第4期451-453,i0002,共4页Medical Journal of Wuhan University

基　　金：湖北省科技厅科技攻关项目(编号:2003AA304B15)

摘　　要：目的:通过检测血管内皮生长因子(VEGF)和间质金属蛋白-酶2(MMP-2)及金属蛋白酶组织抑制剂-2(TIMP-2)在肾细胞癌(RCC)中的表达情况以探讨它们在肾癌的发生、侵袭和转移过程中的作用。方法:用免疫组织化学方法检测40例RCC石蜡标本中MMP-2、TIMP-2和VEGF的蛋白表达情况,并进行统计学分析。结果:MMP-2和VEGF在RCC中表达率明显升高,但不同病理分级、临床分期间差异无显著性(P>0.05);淋巴结转移阳性组MMP-2和VEGF的表达率均明显高于淋巴结转移阴性组,两者之间存在显著性差异(P<0.05);TIMP-2与MMP-2表达存在负相关性,TIMP-2对MMP-2有一定拮抗作用。结论:MMP-2与TIMP-2平衡状态的改变,很可能与RCC的侵袭和转移密切相关。VEGF可作为判断RCC淋巴结转移和临床预后的生物学指标之一。Objective： To investigate the expression of vascular endothelial growth factor （VEGF）, matrix metalloproteinases-2（MMP-2）and its inhibitor tissue inhibitor of metalloproteinase-2（TIMP- 2） in renal cell carcinoma（RCC）. Methods： Protein expression of MMP-2 ,TIMP-2 and VEGF in 40 RCC tissues was examined by immunohistochemical assay. Results： MMP-2 and VEGF showed an overexpression rate in RCC, but there were no significant differences among different pathological grades and clinical stages. The expression of MMP-2 and VEGF was significantly different between the metastasis group and the non-metastasis group （P〈0. 05）. A negative correlation was detected between MMP-2 and TIMP-2. TIMP-2 has a inhibitory action on MMP-2. Conclusion： Overexpression of MMP-2 appears to have prognostic significance with respect to the invasion and metastasis of RCC. The effect of MMP-2 can be restrained selectively by TIMP-2. The balance between MMP-2 and TIMP-2 shows an important role in the development and invasion of RCC. VEGF may also serve as a predictor of clinical prognosis and lymph node metastasis.

关 键 词：肾细胞癌 间质金属蛋白酶 金属蛋白酶组织抑制剂 血管内皮生长因子 

分 类 号：R737.11[医药卫生—肿瘤]