术前胃动脉化疗栓塞对胃癌淋巴结转移灶癌细胞凋亡的影响  

Effect of Preoperative Gastric Arterial Chemoembolization on Apoptosis of Lymph Node Metastasis of Gastric Cancer

作　　者：蒲青凡[1] 孙碎康[1] 刘占培[2] 严律南[2] 蔡宇[1] 曹高健[1] 吴文珍[1] 虞希祥[1] 

机构地区：[1]温州医学院附属第三医院普外科,温州325200 [2]四川大学华西医院普外科,成都610041

出　　处：《中国普外基础与临床杂志》2006年第4期427-430,共4页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的探讨术前胃动脉化疗栓塞对胃癌淋巴结转移灶癌细胞凋亡的影响。方法对40例胃癌伴淋巴结转移患者行胃癌根治性切除术,根据术前是否行胃动脉化疗栓塞分为治疗组和对照组,各20例,采用免疫组化ABC法检测淋巴转移灶p53、bcl-2及CD95基因的表达,原位末端脱氧核苷转移酶标记法(TUNEL)检测细胞凋亡情况。结果治疗组淋巴转移灶p53和CD95表达较对照组明显增强(P<0.05),并与细胞凋亡呈正相关,bcl-2表达则减低与细胞凋亡呈负相关。结论胃癌术前行胃动脉化疗栓塞,可能通过p53、bcl-2及CD95介导使转移淋巴结癌细胞凋亡增加,有助于提高根治性手术的治疗效果。Objective To study the effect of preoperative gastric arterial chemoembolization on apoptosis of lymph node metastasis of gastric cancer. Methods Forty patients with gastric cancer and lymph node metastasis underwent curative resection, among which there were 20 patients who received the preoperative gastric arterial chernoernbolization, and they constituted the treatment group. The rest of the patients were included in the control group. The expressions of p53, CD95 and bcl-2 were examined by immunohistochemistry and apoptosis in the lymph node metastasis was examined by in situ terminal transferase-mediated dUTP nick end labeling （TUNEL）. Results The expression intensity of p53 and CD95 in lymph node metastasis of treatment group increased more significantly than that of control group, whereas the expression intensity of bcl-2 decreased in treatment group. There was a significantly positive correlation between the expressions of p53 and CD95 and the apoptosis. Conclusion Preoperative gastric arterial chemoembolization may affect the expressions of p53, CD95 and bcl-2 and may induce the apoptosis of lymph node metastasis. It may be helpful to improve the effect of curative resection of gastric cancer.

关 键 词：胃癌 转移 化疗栓塞 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]