5-LOXmRNA和COX-2mRNA在胰腺癌中的表达及其临床意义  

The expressions of 5-LOX mRNA and COX-2 mRNA in pancreatic cancer and their clinical significance

作　　者：吕丽红[1] 张波[2] 曾庆东[3] 徐霞 

机构地区：[1]山东大学医学院手术学研究室,山东济南250012 [2]山东省肿瘤防治研究院肝胆外科,山东济南250117 [3]山东大学齐鲁医院普通外科,山东济南250012 [4]山东省建筑医院,山东济南250031

出　　处：《中国普通外科杂志》2006年第6期419-422,共4页China Journal of General Surgery

摘　　要：目的研究5脂氧合酶(5-LOX)在胰腺癌中的表达,探讨其与环氧合酶2(COX-2)的关系。方法用RT-PCR检测5-LOXmRNA和COX-2mRNA基因在3 5例胰腺癌新鲜组织中的表达。结果5-LOXmRNA和COX-2mRNA在胰腺癌组织中的表达率分别为7 4.3%及8 0.0%。两者无协同作用(P>0.0 5)。5-LOXmRNA与胰腺癌的TNM分期有关,III,IV期5-LOXmRNA表达明显高于I,II期(P<0.0 5)。结论5-LOXmRNA和COX-2mRNA在胰腺癌中表达增高,与临床分期有关。两基因的表达无相关性。Objective To investigate the expression of 5- LOX in pancreatic cancer tissue and the relationship between expression of 5-LOX and COX-2. Methods The expression of 5-LOXmRNA and COX-2mRNA in 35 fresh pancreatic cancer tissue samples were detected by semi-quantitive reverse transcriptase-polymerase chain reaction method. Results Expression of 5-LOX mRNA and COX-2mRNA in pancreatic cancer tissue was 74. 3% , 80% , respectively. Expressions of 5-LOX mRNA and COX-2 mRNA were not coordinated （ P 〉0.05 ）. There was correlation between the expression of 5-LOX and TNM stages. The expression of 5- LOX in stages Ⅲ- Ⅳ of pancreatic cancer was markedly higher than that in stages Ⅰ --Ⅱ of pancreatic cancer（ P 〈 0. 05 ） . Conclusions Expression of 5-LOX and COX-2 in pancreatic cancer tissue was markedly elevated, and correlated with clinical stage. Expressions of 5-LOX and COX-2 in pancreatic cancer were not coordinated.

关 键 词：胰腺肿瘤/诊断 RNA 信使/遗传学 5脂氧合酶 环氧合酶2 

分 类 号：R735.9[医药卫生—肿瘤] Q58[医药卫生—临床医学]