咬合力丧失影响大鼠牙周组织中iNOS mRNA的表达  

Effects of bite force lost on the expression of iNOS mRNA in the rat periodontium

作　　者：李晶[1] 洪岩松[1] 钟鸣[1] 艾红军[1] 

机构地区：[1]中国医科大学口腔医院,沈阳110002

出　　处：《现代口腔医学杂志》2006年第4期394-396,共3页Journal of Modern Stomatology

基　　金：辽宁省自然科学基金资助项目(编号:20032055)

摘　　要：目的研究在体内咬合力丧失情况下iN0s影响牙周组织改建的分子机制。方法选用81只Wistar雄性大鼠(体重250±20g)建立正常咬合力、咬合力丧失的动物模型,按照正常、拔牙后6小时、1天、2天、3天、1周、2用、3用、4周随机分组,采用HE染色和RT-PCR的方法,观察牙周细胞中iNOS及iNOSmRNA表达的动态变化。结果组织形态学观察到实验组的牙用组织较对照组有明显改建:牙周膜结构由致密变得稀疏。纤维、细胞的排列由有序到紊乱,牙槽骨骨壁由平整变得凹凸不平,甚至出现了破骨细胞等。RT-PCR蛄果显示咬合力丧失6小时后iNOSmRNA的表达即有增强并在第2天时达到第一个表达高峰,随后逐渐减少。至第l用时已低于正常,然后又开始增加。在第3用时逸到最高峰,之后持续保持高于正常水平,统计学分析差异性显著(P<0．01)。结论咬合力丧失促使牙周细胞产生iNOSmRNA明显增多且呈一定的规律性变化。导致牙周组织发生了一系列的退行性改变。提示iNOS很有可能是影响牙用组织改建的一个重要因素。Objective To establish iNOS for the study of molecular mechanism of the effect of bite force loss on periodontium remodeling. Methods Eighty - one male Wistar rats（weigh 250 ± 20g） were chosed to establish rat models of different bite force for the study, and left maxillary molars of rats were extracted and left mandibular molar area was used as the model with bite force lost. The animals were sacrificed after 6 hours, 1,2,3 days and 1,2,3,4 weeks. The prepared tissue specimens were processed for the study of the change in the histologic morphology and the expression of iNOSmRNA in PDLC and osteoblasts with HE staining and RT - PCR techniques. Results In the group of lost biting force, the derangement of periodontal ligament and resorption of alveolar bone were observed in histologic study： the structure of periodontal ligament deranged , fibers and cells arrayed disorderly, there were many holes in the alveolar bone, occasionally occurred osteoclasts; the expression of iNOSmRNA was observed dramatically changed： the expression of iNOSmRNA in periodontal ligament fibroblasts and osteoblasts was higher than normal, especially at 2d and 3w. Conclusion Bite force lost induce the expression of iNOSmRNA enhanced apparently in PDLC and osteoblasts, it suggestes that iNOS may play important roles in the process of periodontium remodeling.

关 键 词：咬合力丧失 INOSMRNA 牙周组织改建 

分 类 号：R78[医药卫生—口腔医学]