利用siRNA抑制人骨肉瘤细胞survivin的表达  

Inhibition of the Expression of survivin in Osteosarcoma Cell by A Short Hairpin RNA

作　　者：李鲲[1] 杨述华[1] 刘红云[2] 傅德皓[1] 宁旭[1] 梅荣成[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院骨科,武汉430022 [2]青岛市立医院病理科

出　　处：《肿瘤防治研究》2006年第7期519-521,F0003,共4页Cancer Research on Prevention and Treatment

摘　　要：目的构建生存素(survivin)短发夹状RNA表达载体,检测其对骨肉瘤细胞survivin mRNA及蛋白表达的影响。方法体外构建survivin shRNA表达载体pSilence2.1-neo-survivin,转染骨肉瘤细胞系MG-63,RT-PCR、免疫组化方法检测转染前后survivin mRNA及蛋白的表达,MTT比色法检测细胞增殖活性。结果pSilence2.1-neo-survivin载体转染能显著抑制survivin mRNA、蛋白表达,转染后48h细胞增殖的抑制率为61.83%。结论pSilence2.1-neo-survivin可显著抑制MG-63细胞survivinmRNA、蛋白的表达及细胞的增殖活性。Objective To construct a expression plasmid of a short hairpin RNA targeting survivin, and investigate its inhibitory effect on the expression of survivin in osteosarcoma cell line MG-63 in vitro. Methods Construct a expression plasmid of a short hairpin RNA targeting survivin, and transfect it into MG-63 cell by dosper liposomal. The expression of survivin mRNA and protein were examined by RTPCR and immunohistochemical method, respectively. The inhibition of the cell proliferation was estimated by MTT method, Results survivin shRNA could significantly reduce the expressions of survivin mRNA and protein. Survivin shRNA also can inhibit the growth of the cell by MTT method. The inhibition rate reach 61, 83% at 48h after transfection. Conclusion Survivin shRNA could significantly reduce the expressions of survivin mRNA and protein and inhibit the proliferation of the MG-63 cell.

关 键 词：SURVIVIN RNA干扰 短发夹RNA 骨肉瘤 

分 类 号：R738.1[医药卫生—肿瘤]