皮质发育障碍模型的建立及其致敏感性的研究  

Modeling of Cortical Dysplasia and Study of Seizure Susceptibility in This Model

作　　者：龙莉莉[1] 肖波[1] 王康[1] 

机构地区：[1]中南大学湘雅医院神经内科,410008

出　　处：《中国临床神经科学》2006年第4期343-347,共5页Chinese Journal of Clinical Neurosciences

基　　金：教育部高等学校优秀青年教师教学科研奖励计划基金资助项目(2001-182);高等学校博士学科点专项科研基金(2003-172)

摘　　要：目的:建立皮质发育障碍模型,探讨皮质发育障碍模型的敏感性。方法:在SD大鼠孕17d腹腔注入1,3-二氯乙烯-亚硝基脲(BCNU)制作皮质发育障碍模型;Nissl染色观察P60d仔鼠病理变化;选取P60d雄性仔鼠,腹腔注射氯化锂-毛果芸香碱,分别比较两组大鼠癫发生的潜伏期、持续状态时间和死亡率。结果:同龄仔鼠脑组织湿重实验组比对照组显著减轻(P<0.01);Nissl染色显示皮质变薄、皮质层次紊乱、海马区域异位细胞异常聚集;有皮质发育障碍的仔鼠注射氯化锂-毛果芸香碱后,癫发生的潜伏期显著缩短(P<0.01),癫持续状态时间延长(P<0.01),死亡率显著升高(P<0.05)。结论:BCNU致皮质发育障碍模型具有癫易感性。Aim： To establish a model of cortical dysplasia, and to analysis the seizure susceptibility in this model. Methods： Pregnant rats were given intraperitoneal injections of BCNU on embryonic day 17（E17）. Cresylviolet staining was applied to observe the histologic alterations in the resulting pups at P60. P60 male rats were injected with LiCl-pilocarpine intra-peritoneally and then compare the latency to seizure onset, duration of SE （Epileptic status） and mortality in two groups. Results： The mean wet brain weight in the BCNU-exposed pups were lower than those of controls at the same age; cresyl-violet staining revealed disruption of cortical lamination, heterotopic cell clusters within the hippocampus; after injecting pilocarpine intra-peritoneally, adult rats with cortical dysplasia had shorter latency to sezure, longer duration of SE and higer mortality compared to age-matched controls. Conclusion： Seizure susceptibility is increased in the BCNU exposed animal model of cotical dysplasia.

关 键 词：1 3-二氯乙烯-亚硝基脲 皮质发育障碍 氯化锂-毛果芸香碱 癫痫易感性 

分 类 号：R742.1[医药卫生—神经病学与精神病学]