丘脑底核高频电刺激对大鼠黑质-纹状体多巴胺代谢的影响  

Effects of STN-HFS on Substantia Nigra and Striatum Dopaminergic Metabolism in Rats

作　　者：赵永波[1] 王乔树[1] 孙伯民[2] 郭春妮[1] 

机构地区：[1]上海交通大学第一人民医院神经内科,上海200080 [2]上海交通大学医学院瑞金医院神经外科

出　　处：《上海交通大学学报（医学版）》2006年第7期725-729,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(30270493);上海市引进海外高层次留学人员专项基金(20040115);上海市重点基金(024119037)资助项目

摘　　要：目的研究丘脑底核(STN)高频电刺激(HFS)对大鼠黑质-纹状体系统的影响。方法给予正常大鼠一侧STN-HFS,应用微透析观察其对纹状体多巴胺(DA)及其代谢产物的影响,应用逆转录-聚合酶链反应(RT-PCR)和W estern b lot观察其对黑质、纹状体酪氨酸羟化酶(TH)的影响。结果刺激侧纹状体DA代谢产物明显增多(P<0.05),DA水平无变化;RT-PCR检测发现刺激侧黑质、纹状体TH mRNA水平升高;W estern b lot检测发现刺激侧黑质TH表达无变化,而纹状体TH表达明显升高(P<0.01)。结论STN-HFS可能通过影响黑质-纹状体DA代谢及其限速酶的水平发挥作用。Objective To identify the mechanisms of the subthalamic nuclei （STN） high frequency stimulation （HFS） for Parkinson＇ s disease （PD）. Methods We investigated the effects of STN-HFS on striatal dopaminergic metabolism using in vivo microdialysis in the freely moving rats. Furthermore, reverse transcription-polymerase chain reaction and Western blot of striatal and nigral tyrosine hydroxylase （TH） were performed. Results STN-HFS increased striatal dopaminergic metabolism and enhanced striatal TH protein and gene expression without affecting TH protein expression in substantia nigra. Conclusion The application of STN-HFS could compensate for the dopamine deficit by inducing increased striatal dopamine metabolism and modificating protein or gene expression of the rate-limiting enzyme.

关 键 词：帕金森病 高频电刺激 酪氨酸羟化酶 微透析 丘脑底核 

分 类 号：R742.5[医药卫生—神经病学与精神病学]