口腔唾液中一氧化氮的荧光分光光度法检测  

A fluorometric assay for the measurement of Cnitric oxide in human saliva

作　　者：唐卫忠[1,2] 林珠[1,2] 段银钟 孙淑芬[1,2] 王吉村 

机构地区：[1]西安市第四军医大学 口腔医学院 化学教研室 [2]西京医院

出　　处：《牙体牙髓牙周病学杂志》1996年第3期129-132,共4页Chinese Journal of Conservative Dentistry

摘　　要：目的：对健康人口腔唾液中一氧化氮（Ｎｉｔｒｉｃｏｘｉｄｅ，ＮＯ）的含量进行检测，初步探讨唾液中ＮＯ的作用，并为更深入地研究唾液ＮＯ提供方法上的尝试。方法：采用经改良的Ｍｉｓｋｏ荧光分光光度法检测了７４名健康人口腔唾液中ＮＯ－２的含量，以间接反映ＮＯ的含量。结果：７４名健康人唾液中ＮＯ－２含量无性别差异，平均为３．０７１±０．５５７μｍｏｌ／Ｌ；随着年龄的增加，ＮＯ－２含量有明显增加的趋势。结论：唾液中ＮＯ含量可随组织增龄性的变化而增加；此外，该荧光分光光度法对唾液中ＮＯ的检测较为灵敏、精确。Aim: To study the effects of NO in human saliva, and try to find a new approach available to study NO in human saliva. Methods: Using an improving fluorometric method for the determination of nitrite, which stands for the content of nitric oxide. Results: Mean level of NO in saliva of 74 healthy individuals was 3.071±0.557μmol/L,and there was no significant difference between male and female. There was a relationship between the increasing of salivary NO and aging. Conclusions: The level of NO in saliva was increased with aging. NO in saliva may play a role in the maintenance of mucosal and periodontal ligament integrity, and this fluorometric method may be a simple specific and sensitive method for the measurement of nitrite in human saliva.

关 键 词：唾液 一氧化氮 荧光分光光度法 口腔疾病 

分 类 号：Q592.9[生物学—生物化学] R780.2[医药卫生—口腔医学]