化疗对结核病大鼠T细胞亚群变化的影响  

The effect of the anti-tuberculosis chemotherapy to the T lymphocytes subset of the rats with tuberculosis

作　　者：雷建平[1] 彭燕[2] 熊国亮[1] 张玉珍[3] 周军[1] 刘翼军[1] 邢娟娟[2] 李光明[1] 雷铭宙[1] 袁小亮 涂荣耀[1] 涂少华[1] 

机构地区：[1]江西省胸科医院,南昌330006 [2]南昌大学第一附属医院流式细胸室,南昌330006 [3]南昌大学医学院动物科学部,南昌330006 [4]不详

出　　处：《江西医药》2006年第6期361-364,共4页Jiangxi Medical Journal

基　　金：江西省社会发展攻关重点基金;江省卫生厅重大招标课题

摘　　要：目的探讨CD4+CD8+双阳(DP)T细胞与结核病相关性。方法60只PPD皮试阴性SD大鼠随机分成(1)结核病化疗组、(2)感染对照组、(3)健康对照组。化疗组和感染组大鼠均于观察开始接受结核菌标准株H37Rv(TB)攻击,攻毒1个月后化疗组接受抗TB化疗;三组动物均于感染结核分枝杆菌前和感染后1、2、3、5个月末检测外周血中CD3+、CD4+、CD8+、DPT细胞含量。结果5个月全程观察中,感染后1个月内化疗组、感染对照组各死亡2例(10%);2～5个月内化疗组死亡率5.6%(1/18),不化疗组同期死亡率38.9%(7/18),P<0.05;健康对照组动物无死亡。开始观察时3组之间各对应参数对比差异无显著性,P>0.05;对照组观察期内各参数的改变差异亦无显著性,P>0.05;感染1个月后,化疗组、感染对照CD4+单阳(SP)细胞数、CD4+/CD8+T细胞比率降低,而CD8+、DPT细胞数升高,P<0.05或P<0.01。不化疗的感染对照组在感染后1个月时与其后2～5个月各参数差异无显著性,P>0.05。化疗组化疗1个月后各参数恢复到结核感染前相近水平,与对照组各对应参数差异已无显著性,P>0.05。TB感染后,CD4+单阳、CD4+/CD8+比率降低,而DP、CD8+T细胞升高呈负相关。结论CD4+CD8+双阳(DP)T细胞是抗结核保护性免疫相关细胞。Objective To study the effect of the anti-tuberculosis chemotherapy （anti-TB） to the T lymphocytes subset of the rats with tuberculosis. Methods 60 SD big rat with PPD skins-test negative were divided into： （1） the Anfi-TB group, （2） Tuberculosis （not anfi-TB） group,（3）Control group. All rats of the Anti-TB group and Tuberculosis group were inoculate a dose of l0s CFU viable Mycobacterium tuberculosis at the beginning of the observation. After one month, the rats in anti-TB group accept anti-tuberculosis chemotherapy. Observing death rate of each set; examine CD3^＋, CD4^＋, CD8^＋, CD4^＋CD8^＋ double positive （DP） T cell in peripheral blood at the experiment start and end of behind 1, 2, 3, 5 months. Results （1） There were two rats died in the anti-TB group and tuberculosis group in the first month respectively;Mortality of the anti-TB group during the chemotherapy was 5.6% （1/18）, lower than that of the not anti-TB group（7/18, 38.9%）, P〈0.05. There were not rats died in the control group. （2） The tuberculosis rats expressed that CD4/CD8 and CD4^＋ simple positive（SP） T lymphocytes reduced, at the same time CD8^＋ SP T lymphocytes and DP T lymphocytes went up, P〈0.05 or P〈0.01.These changes can recover to near the normal level in 1 or 2 months for chemotherapy. Conclusion DP T cells is a cell set related the immunity of anti-tuberculosis.

关 键 词：结核病 细胞免疫学 CD4^+CD8^+ 双阳T淋巴细胞 卡介苗 

分 类 号：R-332[医药卫生]