早熟凝集染色体和诱导凝集的染色体扫描电镜研究  

Studies on Prematurely Condensed Chromosomes and Induced Condensed Chromosomes with SEM

作　　者：潘岛[1] 刘凌云[1] 

机构地区：[1]北京师范大学生物系,美国Minnesota大学

出　　处：《Acta Genetica Sinica》1996年第6期425-430,共6页

基　　金：国家自然科学基金

摘　　要：应用细胞融合技术和扫描电镜研究了ＢＫ（牛肾）细胞早熟凝集染色体（ＰＣＣ）和诱导ＰＣＣ的ＣＨＯ中期染色体的超微结构。所用电镜标本制备方法，尽可能使之减少人工效应，故首次观察到ＰＣＣ超微结构，特别是早、中、晚Ｓ－ＰＣＣ超微结构的自然状态，从早、中、晚Ｓ－ＰＣＣ的染色体片段所显示的ＰＣＣ的多级亚结构，进一步了解了ＢＫ细胞染色体的超微结构。诱导ＰＣＣ的ＣＨＯ中期染色体表面显示出整齐的小毛样由螺旋纤维形成的环状突起。The ultrastructure of prematurely condensed chromosomes (PCC) in BK cells and the CHO metaphase chromosomes (the PCC inducer) were studied with cell fusion technique and SEM. The methods for prepareing complete specimens for SEM, were used to eliminate artifact as complete as possible, so the natural states of PCC ultrastructure, especially the ultrastructure of the early, mid, and late S-PCC,were observed for the first time. In the three phases of S-PCC multiple double strand helical structure were revealed,on the chromosome segments of BK cells. On the surface of CHO metaphase chromosomes which may induce PCC may regularly arrayed short hair-like-loop-protrusions consisting of helical chromatin fibers were obserred.

关 键 词：染色体 早熟凝集染色体 细胞融合 扫描电镜 

分 类 号：Q343.23[生物学—遗传学]