活络健骨胶对大鼠佐剂性关节炎的抗炎免疫效应  

Anti-inflammatory and immune effects of Huoluojiangu capsule on adjuvant arthritis in rats

作　　者：周家茂[1] 严奉祥[2] 雷黎明[1] 胡志祥[1] 李葵香[1] 余平[1] 周小兵[2] 谢蓝[1] 陈羿汐[1] 聂兰秀[1] 

机构地区：[1]湖南环境生物学院医学部,湖南省衡阳市421001 [2]南华大学药理研究所,湖南省衡阳市421001

出　　处：《中国临床康复》2006年第27期48-50,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察活络健骨胶囊对大鼠佐剂性关节炎的抗炎免疫作用。方法:实验于2005-12-25/2006-01-23在湖南环境生物学院医学部药理实验室完成。①将48只wistar大鼠采用随机数字法分成6组,正常对照组和模型组:均给予等量蒸馏水;阳性对照组:给予雷公藤多甙7mg/kg;活络健骨胶囊高、中、低剂量组:剂量分别为15,10,5g/kg。活络健骨胶囊为老中医祖传经验方(由制川乌、赤芍、桂枝10多味中草药组成,原料药购于衡阳药材公司,鉴定为合格品,实验药液由本实验制备)。造模前3d开始给药,造模后连续给药25d。给药途径为灌胃,给药体积为等容15mL/kg。②建立大鼠后右足佐剂性关节炎模型。③造模后第2,5,10,15,20,25d分别测各鼠右后足容积,计算各组大鼠右后足在不同时间的肿胀度。④每周观察大鼠体质量增长率及造模后26d计算脾、肾上腺指数。增长率=(给药后体质量-给药前体质量)/给药前体质量×100%;器官指数=器官湿重/大鼠体质量×100。⑤观察大鼠佐剂性关节炎渗出液中白细胞介素2,白细胞介素6及前列腺素E2含量。结果:48只大鼠全部进入分析。①活络健骨胶囊对大鼠体质量及脾、肾上腺素的影响:活络健骨胶囊高、中剂量组能抑制造模鼠体质量的减轻(P<0.01);对肾上腺指数有促进作用(P<0.05)。②活络健骨胶囊对大鼠佐剂性关节炎足跖肿胀的影响:活络健骨胶囊各剂量组对造模足跖有抑制作用,而中、高剂量组的抑制作用更显著(P<0.01)。③活络健骨胶囊对佐剂性关节炎渗出液中白细胞介素2、白细胞介素6及前列腺素E2含量的影响:活络健骨胶囊各剂量组对大鼠佐剂性关节炎中白细胞介素6及前列腺素E2的生成均有显著抑制作用(P<0.01),而对白细胞介素2的含量无明显影响。结论:活络健骨胶囊具有良好的抗炎免疫效果,其作用机制之一可能是与抑制单核吞噬细胞的激活、分化及介导�AIM： To study the anti-inflammatory and anti-immune effects of Huoluojiangu capsule （HJC） on the rats with adjuvant arthritis. METHODS： The experiment was accomplished in the Laboratory of Pharmacology, Medical Department of Hunan Environment and Biology College from December 25^th 2005 to January 23^rd 2006.①Forty-eight Wistar rats were randomly assigned info 6 groups： normal control group and model group： Both were treated with equal distilled water; positive control group： 7 mg/kg multi-Glycoside common threewingnut root; HJC groups of high, medium and low dose： HJC at the dosages of 15, 10 and 5 g/kg. （HJC containe.d more than ten kinds of herbs, such as radix aconiti praeparata, red peony and cassia twig rough. All the crude drugs were purchased from Hengyang Medicinal Material Company, and the experimental liquid was prepared by this laboratory.） The administration procedure was performed continuously from three days before modeling to 25 days after modeling by intragastric infusion at the total volume of 15 mL/kg. ②The arthritis models was established in the right feet of rats induced by Freund＇s complete adjuvant. ③The weight and the swelling degrees of right hiridfeet were measured at different time points （2, 5, 10, 15, 20 and 25 days after modeling）.④The growth rates of rat weight were observed every week, and the adrenal gland and spleen in rats were also calculated on the 26^th day after modeling. Growth rate =（pretreatment weight-post-treatment weight）/pretreatment weight×100%; Organ index--wet weight/weight of rat×100.⑤The contents of prostaglandin E2 （PGE2）, interleukin-2 （IL-2） and IL-6 were determined in the exudates of adjuvant arthritis rats. RESULTS： Totally 48 rats were involved in the result analysis.①Effects of HJC on the weight, spleen and adrenal gland of rats： HJC of high and medium dosages could inhibit the decreasing weight （P 〈 0.01） and promote the adrenal gland index （P 〈 0.05）.②Effects of HJC on the swelling

关 键 词：中药疗法 关节炎 实验性 佐剂 免疫 

分 类 号：R749.611[医药卫生—神经病学与精神病学]