水溶性壳寡糖对人白血病细胞诱导分化的影响  

The effect of differentiation on induction in human leukemia cells by chitooligosaccharide

作　　者：徐广飞[1] 李春建[2] 仲齐庆[2] 杨宇民[3] 

机构地区：[1]南通大学公共卫生学院营养与食品卫生学教研室,南通22600 [2]南通大学公共卫生学院公共卫生学院实验室,南通22600 [3]南通大学公共卫生学院神经再生重点实验室,南通22600

出　　处：《南通大学学报（医学版）》2006年第4期247-248,共2页Journal of Nantong University(Medical sciences)

摘　　要：目的:研究水溶性壳寡糖(WSC)抑制人白血病细胞(HL-60)增殖活性的作用。方法:将WSC与HL-60细胞共培养,四甲基偶氮唑盐(MTT)比色法检测WSC对HL-60细胞增殖的抑制作用;氮蓝四唑(NBT)法检测WSC对HL-60细胞分化促进作用;流式细胞仪定量检测细胞凋亡。结果:250～1000mg/L的WSC能抑制HL-60细胞增殖,并促进HL-60细胞分化,WSC作用48h后流式细胞仪分析图上出现凋亡峰。Objective ：To expolore the effect of water- soluble chitooligosaccharide（ WSC ） on induction of differentiation in human leukemia HL -60 cells. Methods ：The inhibitory effect of WSC on growth of HL -60 was measured by MTT assay, and induction of WSC on differentiation of HL -60 was assayed by nitroblue tetrazolium method. Flow cytometry was used to analyze apoptosis qualitatively anti quantitatively. Results：250 - 1 000mg/L WSC inhibited the growth of HL - 60 cells. This effect was accompanied by a marked increase in the proportion of G0/G1 cells as measured by flow cytometry. WSC also induced differentiation of the HL - 60 cells as measured by phorbol ester - dependent reduction of nitroblue tetrazolium. Conclusion：WSC can induce apoptosis and differentiation in human leukemia HL- 60 cells .

关 键 词：人白血病细胞 水溶性壳寡糖 细胞分化 细胞凋亡 

分 类 号：R733.7[医药卫生—肿瘤]