两种蚂蚁巢甲醇提取物的自由基清除及抗菌活性研究(英文)  

Radical Scavenging and Antimicrobial Activities of Methanol Extracts of Two Kinds of Formicary

作　　者：李建许[1] 徐学清[1] 杨海龙[1] 刘彤光[2] 赖仞[1] 

机构地区：[1]中国科学院昆明动物研究所 [2]青岛大学医学院附属医院,青岛266003

出　　处：《中国天然药物》2006年第4期295-298,共4页

基　　金：国家自然科学基金资助项目(30200044/C01040101)~~

摘　　要：对两种不同的蚂蚁窝进行甲醇提取，并对提取物进行了急性毒性、抗自由基，酚含量、还原能力和抗菌活性的检测。二苯基三硝基肼（1，1-diphenyl-2-picrylhydrazyl，DPPH）自由基的清除能力的检测表明：两种蚂蚁窝甲醇提取物都具有显著的自由基清除能力，并呈现出剂量依赖性，而且，在高浓度时（5～10mg·mL^-1），它们的自由基清除能力甚至显著地高于ST基羟基甲苯（butylated hydroxyl toluene，BHT）。酚的含量和还原能力分别用福林酚试剂和铁氰化钾还原反应来测定，统计分析显示两种蚂蚁窝的抗氧化能力和其酚含量、还原能力都存在着正相关。两种蚂蚁窝甲醇提取物都具有抗革兰氏阴性菌和革兰氏阳性菌的活性，但它们却对真菌没有作用。Methanol extracts of two kinds of formicary were investigated for their free radical scavenging activities, total phenolic content, reduction power and antimicrobial activities. Both of them showed no toxic effect on mice at the testing dose of 10 mg/kg （p. o）. The 2,2-diphenyl-1-picrylhydrazyl （DPPH） free radical scavenging test showed that both of the formicary extracts were potent radical scavengers and their scavenging activities were dose- dependent. Furthermore, at higher concentrations （5 - 10 mg/ml） both of them exhibited significantly higher DPPH scavenging activity than butylated hydroxytoluene （BHT）. Total phenolic content and reducing power were determined using the Folin-Ciocalteu and the potassium ferricyanide reduction methods, respectively. Statistical analysis indicated a close association between the antioxidant potency and total phenolic content, and between the antioxidant potency and reducing power as well. Both methanol extracts presented antimicrobial activities on gram - negative and gram-positive bacteria. However, they were inactive on fungus .

关 键 词：自由基清除能力 还原能力 酚含量 抗菌活性 

分 类 号：R965[医药卫生—药理学]