β-内啡肽在高功率微波辐射后大鼠海马神经元中的表达及其意义  

Expression and significance of expression of β-endorphin in hippocampus after HPM radiation in rat

作　　者：吴振宇[1] 徐天昊[1] 彭瑞云[1] 杨瑞[1] 高亚兵[1] 王水明[1] 胡文华[1] 马俊杰[1] 杨怡[1] 杨国山[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《解放军医学杂志》2006年第7期686-688,共3页Medical Journal of Chinese People's Liberation Army

基　　金：全军杰出人才基金课题(04J016);国家863课题资助项目(30300180)

摘　　要：目的 探讨高功率微波（HPM）辐射后大鼠海马神经元中β-内啡肽的表达及其意义。方法 采用12mW／cm^2HPM模拟源辐照50只Wistar雄性大鼠，于照射后6h、1、3、7、14、28天活杀取大脑海马组织，经光镜、电镜、免疫组化和图像分析等技术，研究HPM辐射后大鼠海马损伤的特点、β-内啡肽的表达及其意义。结果HPM辐射后6h，海马神经元线粒体肿胀，髓鞘融合、解离。照射后3天神经元线粒体肿胀、空化，嵴断裂，核染色质浓缩、边集，突触间隙不清，囊泡堆积或排空。照射后1-7天，神经元胞浆中争内啡肽表达进行性增加，7天达高峰，14天后逐渐恢复，28天基本恢复至正常水平。结论 HPM辐射可引起海马组织学和超微结构的损伤，而β-内啡肽表达增加，且呈规律性变化，参与了海马损伤与修复的病理生理过程。Objective To investigate the expression and significance of β-endorphin in hippocampus after high power microwave （HPM） radiation. Methods 50 male Wistar rats were sacrificed at 6h, ld, 3d, 7d, 14d, 28 days after exposure to 12rnW/cm2 HPM imitational source radiation. The hippocampus was harvested, the characteristics of injury to the hippocampus and expression of ~endorphin were evaluated by means of light microscope, EM, immunohistochemistry and image analysis. Results In the neutrons of hippocampus, mitochondrial swelling and myelin sheath confluence and dissociation were observed 6 hours after radiation, and mitochondrial swelling, cavitation, disruption of crista, concentration margining of chromatin, blurring of synaptic cleft, piling or evacuation of vesicles were observed 3 days after HPM radiation. The expression of β-endorphin in cytoplasm of neurons was up-regulated continuously from 1 to 7 days, peaking at 7 days, restoring to normal level from 14 to 28 days after radiation. Conclusion HPM can produce injury to the hippocampus at histological and ultrastructural levels. The expression of β-endorphin is up-regulated, and it might play an important role in the pathophysiological process of injury and repair in the hippocampus.

关 键 词：微波 海马 超微结构 Β内啡肽 

分 类 号：R363[医药卫生—病理学]