树突状细胞表面HSP70受体介导的内化作用  

Internalization mediated by HSP70 receptors expressed on the surface of dendritic cells

作　　者：葛伟[1] 隋延仿[1] 孙玉静[1] 曹云新[2] 司少艳[1] 张秀敏[1] 

机构地区：[1]西安第四军医大学基础医学部病理学教研室,710032 [2]西安第四军医大学基础医学部免疫学教研室,710032

出　　处：《解放军医学杂志》2006年第7期689-691,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助项目(30271464)

摘　　要：目的研究树突状细胞(DCs)表面HSP70受体介导的内化作用。方法实验分为3组:HSP70受体封闭组、非受体封闭组和对照组,采用流式细胞仪检测C57BL/6小鼠骨髓来源DCs内化FITC标记的HSP70阳性细胞率。结果受体封闭组50μgHSP70可使DCs表面受体饱和;受体饱和后随HSP70-FITC用量的升高,HSP70-FITC阳性细胞数呈正比性增高;非受体封闭组应用50μgHSP70-FITC与100μgHSP70-FITC时HSP70-FITC阳性细胞率均达到90%以上且组间差别不大。结论DCs表面HSP70受体介导的内化作用是DCs摄取抗原的主要途径,且具有饱和性和优先性的特点。当受体饱和后,非受体介导的内化作用可能在DCs内化HSP70过程中起作用并且呈剂量依赖性。Objective To study the imemalization mediated by heat shock protein 70 （HSI〉70） receptors expressed on the surface of dendritic cells （DCs）. Methods DCs from C57BL/6 mouse bone marrow were divided into HSP70 receptor-blocking group, non-receptorblocking group and control group. The HSP70-FITC positive cells were detected by flow cytometry （FCM）. Results FCM indicated that in HSP70 receptor-blocking group, the HSP70 receptors could be almost totally blocked by 50gg HSPT0. With the increase of HSP70FITC dosage, the number of HSP70-FITC positive cells increased. In non-receptor-blocking group, the rates of the HSP70-FITC positive cells were up to 90% either when 50μg HSP70-FITC or 100μg HSP70-FITC was used with no remarkable difference between them. Conelusions The internalization mediated by HSP70 receptors plays a major role in the stage of ingestion of HSP70 with the characteristics of saturation and priority. Once the receptors are blocked, non-receptor pathway participates in internalization in a dose-dependent manner.

关 键 词：热休克蛋白质70 树突细胞 内化 

分 类 号：R332[医药卫生—人体生理学]