Bcl-2反义核酸增强青蒿琥酯诱导K562细胞凋亡  

Bcl-2 antisense oligodeoxynucleotides to enhance the apoptosis-inducing effect of artesunate on K562 cells

作　　者：赵新汉[1] 王志宇[1] 李晶[1] 全平[1] 

机构地区：[1]西安交通大学医学院第一附属医院肿瘤内科

出　　处：《第四军医大学学报》2006年第14期1282-1285,共4页Journal of the Fourth Military Medical University

摘　　要：目的:研究bcl2反义核酸增强青蒿琥酯对人慢性粒细胞白血病K562细胞株诱导凋亡效应.方法:合成bcl2反义核酸(ASON)导入K562细胞,应用Westernblot法检测bcl2蛋白的表达,TUNEL技术、流式细胞仪法(FCM法)检测细胞凋亡.结果:bcl2反义核酸(ASON)能明显抑制bcl2蛋白的表达.青蒿琥酯在1～7mg/L浓度范围内能分别明显抑制K562细胞的增殖,并具有时间和浓度依赖性,7mg/L青蒿琥酯作用72h的K562细胞株A值最低.原位细胞凋亡检测可见细胞变小,核固缩为一个或多个染色质团块,与未经ASON转染的K562细胞株相比,经bcl2ASON转染的K562细胞株对青蒿琥酯的诱导凋亡作用更加明显;FCM法出现低于G1期DNA含量的亚二倍体凋亡峰,细胞凋亡主要发生在G1/S期,与未经ASON转染的K562细胞株相比,经bcl2ASON转染的K562细胞凋亡率显著增加.结论:青蒿琥酯在体外一定浓度范围内可诱导K562细胞株凋亡,bcl2反义核酸可增强青蒿琥酯诱导K562细胞凋亡的作用.AIM： To study whether bcl-2 antisense oligodeoxynucleotides（ASON） can enhance the apoptosis-inducing effects of artesunate on human chronic granulocytic leukemia K562 cell strain. METHODS： Bcl-2 ASON was synthesized and tmnsfected into K562 cells with liposome, and its expression was detected by Western blotting method. The terminal deoxynucleotidyl transferase mediated dUTP nick end labeling （TUNEL） and flow cytometry （FCM） were applied to detect cell apoptosis. RESULTS： Bcl-2 ASON could obviously silence bcl-2 expression in K562 cell strain. Within the range of 1-7 mg/L, artesunate obviously inhibited K562 cell proliferation in a time- and dose-dependent manner, and 7 mg/L artesunate which affected K562 cells for 72 h had the lowest A value. TUNEL showed that K562 cells became smaller and the nuclei presented one or many condensed chromatin masses, compared to K562 cells which were not transfected with bcl-2 ASON, bcl-2 ASON transfected K562 cells were more sensitive to artesunate; the apoptosis peak of lower sub-diploid DNA content in G1 phase was observed in FCM, and apoptosis of K562 cells induced by artesunate mainly occurred in G1/S phase; compared to K562 cells which were not transfected with bcl-2ASON, bcl-2 ASON-K562 cell apoptosis rate was significantly higher. CONCLUSION： Artesunate could induce the apoptosis of K562 cells within a certain dose range in vitro, and bcl-2 ASON could enhance the apoptosis-inducing effect of artesunate.

关 键 词：青蒿琥酯 基因 BCL-2 RNA 反义 K562细胞 细胞凋亡 

分 类 号：R733[医药卫生—肿瘤]