重组人生长激素作用下成骨细胞细胞周期及细胞骨架改变的体外研究  

Impact of recombinant human growth hormone on cell cycle and cytoskeleton of rat calvarial osteoblasts in vitro

作　　者：郭三萍[1] 石勇[1] 李晓红[1] 宋玮[2] 

机构地区：[1]西安交通大学口腔医院修复科,陕西西安710004 [2]第四军医大学口腔医学院口腔内科,陕西西安710033

出　　处：《第四军医大学学报》2006年第14期1293-1295,共3页Journal of the Fourth Military Medical University

基　　金：陕西省科技计划项目(2003K10G78)

摘　　要：目的:观察重组人生长激素(rhgh)对体外成骨细胞(OB)细胞周期及细胞骨架Factin的影响.方法:用浓度为10和200μg/L的重组人生长激素加入大鼠OB培养体系,观察对大鼠OB的细胞周期及细胞骨架的影响.结果:定量分析表明,在rhgh作用下,成骨细胞S期、G2/M期细胞百分比增加,OB的绿色荧光强度、红色荧光强度无显著变化.Factin纤维呈束状平行排列,荧光染色强,分布均匀.结论:重组人生长激素对OB的合成代谢有直接影响,但对细胞骨架无显著影响.AIM： To observe the changes of the cell cycle and cytoskeleton of rat calvarial osteoblasts after stimulated by recombinant human growth hormone （rh-gh）. METHODS： Osteoblastic cells from rats were incubated in the medium supplemented wiht rh-gh at different doses （ 10, 200μg/L）, and then the changes of cytoskeleton and cell cycle were detected. RESULTS： Quantitative analysis showed that osteoblasts were arrested in S phase and G2/M phase, however, green fluorescent density and red fluorescent density （nuclei） were not significantly changed. The filamentous F-actin distributed in bunches and was stained strongly and evenly. The outline of nucleus was clear. CONCLUSION： Rh-gh exerts a direct anabolic effect on osteoblasts, but no obvious effect on cytoskeleton of rat osteoblasts.

关 键 词：成骨细胞 重组人生长激素 细胞周期 细胞骨架 

分 类 号：R783-1[医药卫生—口腔医学]