中国内陆发现HCoV-HKU1感染及N和S蛋白基因序列及进化分析  

Phylogenetic and Sequence Analyses of the N and S Genes of Coronavirus HKU1,a Novel Human Coronavirus in China's Mainland

作　　者：段招军[1] 黄灿平[2] 瞿小旺[1] 谢志萍[1] 漆正宇[1] 高寒春[2] 彭夫望[1] 刘文培[1] 侯云德[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京100052 [2]病毒生物技术国家工程研究中心,北京100176

出　　处：《病毒学报》2006年第4期241-247,共7页Chinese Journal of Virology

摘　　要：了解冠状病毒HKU1在我国大陆地区的感染情况及N基因和S基因的编码特征。采用RT-PCR的方法对2005年10月～2006年1月收集的260例急性呼吸道感染的住院儿童鼻咽抽吸物（NPA）进行了冠状病毒HKU1基因检测。将PCR阳性产物测序，并将所测序列在GenBank中进行比较分析。260份样本中共检测到2份冠状病毒HKU1阳性，阳性率为0．77％（2／260），且该2例冠状病毒HKU1阳性患者临床均表现为肺炎症状。扩增其中一株病毒N和S全基因，并进行测序，与GenBank中的冠状病毒HKU1参考株及冠状病毒科其它成员进行序列同源性和进化树分析，并对N和S蛋白的结构与功能进行了初步的预测分析。由此表明我国大陆地区存在冠状病毒HKU1感染，且可能与儿童下呼吸道感染存在相关性。HCoV-HKU1, a novel group Ⅱ coronavirus, is associated with community-acquired pneumonia. In order to understand the epidemiological features and the molecular characteristics of HCoV-HKU1 in China's Mainland, RT-PCR assay was applied to investigate HCoV-HKU1 infection in a hospitalized paediatric population with acute respiratory tract infection. Prospectively collected （from October 15, 2005 to January 8, 2006） nasopharyngeal aspirates （NPAs） were subjected to reverse-transcription polymerase chain reaction for detection of HCoV-HKU1 and amplification of the spike （S） and nucleocapsid （N） genes. Two of 260 samples （0.77 % ） tested were positive for HCoV-HKU1. The two HCoV-HKU1-infected patients had pneumonia as clinical symptom. Homologous and phylogenetic analyses revealed the N gene had 99.5% nucleotide identity and 99.8% amino acid identity, the S gene had 99.8% nucleotide identity and 99.9% amino acid identity with HCoVHKU1 prototype. All the above results indicated that HCoV-HKU1 infection exists in China's Mainland and probably has a correlation with lower respiratory tract infection among children.

关 键 词：呼吸道感染 SARS冠状病毒 冠状病毒HKU1 

分 类 号：R373.1[医药卫生—病原生物学]