K252a对腺样囊性癌培养中许旺细胞生长的阻断作用  

The block effect of K252a on the growth of Schwann cell in salivary adenoid cystic carcinoma cell co-culture

作　　者：余立强[1] 孙沫逸[1] 杨耀武[1] 张圃[1] 王平[2] 

机构地区：[1]第四军医大学口腔医学院口腔颌面头颈肿瘤外科 [2]北京军区政治部第四干休所门诊部,北京100036

出　　处：《中国耳鼻咽喉头颈外科》2006年第6期393-395,共3页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：国家自然科学基金资助(30271424);陕西省自然科学基金资助(2002C2-06)

摘　　要：目的观察并探讨K252a在体外坐骨神经长出的许旺细胞对腺样囊性癌细胞趋化生长中的影响。方法采用细胞培养技术,将经神经营养素的高亲和受体Trk特异性阻断剂K252a预处理的小鼠坐骨神经与腺样囊性癌细胞共培养(设24组),以正常小鼠坐骨神经与腺样囊性癌细胞共培养为对照组(设24组);结果与对照组相比,经K252a预处理的坐骨神经与腺样囊性癌细胞共培养长出的许旺细胞增生组数及趋化性生长组数x2值分别为14.03、26.32,P值均小于0.025,说明两组之间的差异非常显著,许旺细胞丧失趋化性及细胞增生未被促进。结论K252a在许旺细胞对腺样囊性癌细胞趋化生长中的阻断作用间接地表明神经营养素可促进许旺细胞增生及向腺样囊性癌细胞趋化迁移,这可能是腺样囊性癌嗜神经性重要机制之一。OBJECTIVE To study the effect of K252a on the chemotactic growth of Schwann Cell to salivary adenoid cystic carcinoma cell in vitro co-culture METHODS Co-culture of sciatic nerve blocked by K252a with salivary adenoid cystic carcinoma cell was regarded as experimental group. Co-culture of sciatic nerve with salivary adenoid cystic carcinoma cell was chosen as control group RESULTS In contrast to control group, the promotion growth and chemotactic growth of Schwann cell was blocked by K252a （P〈0.025）. CONCLUSION The block effect of K252a reveal that the Neurotrophins promote growth of Schwann cell and induce chemotactic growth of Schwann cell. It may be one of the mechanisms of the perineural invasion in salivary adenoid cystic carcinoma.

关 键 词：癌 腺样囊性 许旺细胞 神经营养因子类 坐骨神经 

分 类 号：R73-3[医药卫生—肿瘤]