新生大鼠牙乳头细胞的体外培养和矿化特征  

Culture and the mineralization ability of neonatal rat dental papillae cells

作　　者：方厂云[1] 曹莹[1] 张雪梅[1] 夏宇[1] 李辉莉[1] 苏征[1] 

机构地区：[1]中南大学湘雅医院口腔科,湖南长沙410078

出　　处：《牙体牙髓牙周病学杂志》2006年第7期367-370,共4页Chinese Journal of Conservative Dentistry

摘　　要：目的：观察体外培养的新生大鼠牙乳头细胞的生物学特征。方法：体外培养出生1d大鼠磨牙牙乳头细胞，进行组织学染色，透射扫描电镜观察。结果：体外培养的新生大鼠牙乳头细胞能形成细胞克隆，第3代细胞在含2mmol／L β-甘油磷酸钠、50mg／L抗坏血酸、10^-8moL/L地塞米松的培养液中培养7～8d后可呈现复层生长，培养14～16d后细胞结节中出现矿化，茜素红染色呈阳性反应；透射电镜观察细胞内出现大量含有致密小体的基质小泡样超微结构。结论：发育阶段较早的大鼠牙乳头细胞具有增殖和矿化能力。AIM ： To study the mineralization ability of cultured neonatal rat dental papillae cells （RDPC）. METHODS： Neonatal dental papillae cells were obtained from molar tooth germs of one - day postnatal （ 1 dpn） SD rats and primarily cultured with explant technique in vitro. The cells of the third passage were incubated in the presence of 2 mmol/L β - glyc - erophosphate（ β- GP） , 50 μg/ml L - ascorbicacid（ L - AA） and 10^-8/ml dexamethasone in DMEM containing 15 % FBS. The changes of the cell morphology,ultrastructure and the formation of mineralized nodules were observed by light microscope （LM） and transmission electron microscopy （TEM）. RESULTS ： Cell clones were observed in RDPC monolayer culture in vitro. RDPC presented mineralized nodules as well as matrix vesicles under TEM. CONCLUSION ： The RDPC cultured from one - day postnatal SD rats presented proliferation and differentiation ability in vitro.

关 键 词：牙乳头细胞 细胞培养 细胞分化 

分 类 号：R780.2[医药卫生—口腔医学]