黄芪总苷对血吸虫卵抗原活化的肝星状细胞增殖与胶原合成的影响  

Effect of astragalosides on schistosomal hepatic fibrosis in vitro

作　　者：朱虹[1] 吴强[1] 杨雁[2] 袁小松[3] 汪学龙[3] 沈继龙[3] 

机构地区：[1]安徽医科大学病理学教研室,安徽合肥230032 [2]安徽医科大学药理学教研室,安徽合肥230032 [3]安徽医科大学病原微生物教研室,安徽合肥230032

出　　处：《中国药理学通报》2006年第5期555-558,共4页Chinese Pharmacological Bulletin

基　　金：国家新药基金资助课题(No969010567)

摘　　要：目的观察黄芪总苷对血吸虫卵抗原活化的肝星状细胞增殖与胶原合成的影响。方法小鼠腹腔注射日本血吸虫可溶性虫卵抗原(Solub le egg antigen,SEA),制备SEA活化的腹腔巨噬细胞条件培养基(Solub le egg antigen inducedm acrophage cond itioned m ed ium,SEA-MCM)。采用肝星状细胞株HSC-T6细胞,以细胞增殖抑制率为指标,用噻唑蓝(MTT)测定黄芪总苷对SEA-MCM诱导的HSC-T6细胞增殖的影响;通过3H-脯氨酸参入法测定黄芪总苷对SEA-MCM诱导的HSC-T6细胞胶原合成的影响。结果SEA-MCM能明显促进HSC-T6细胞的增殖和胶原合成。黄芪总苷(32.5、65和130 mg.L-1)对SEA-MCM诱导的HSC-T6细胞增殖及胶原合成有明显的抑制作用,且呈药物浓度依赖性关系。结论黄芪总苷对SEA-MCM诱导的肝星状细胞增殖及胶原合成有明显的抑制作用。Aim To observe the effect of astragalosides on proliferation and collagen synthesis of HSC-T6 cells driven using schistosoma japonicum soluble egg antigen-activated macrophage conditioned medium （SEAMCM）. Methods SEA-MCM was prepared by injection of Schistosoma japonicum SEA via mice peritoneal. The proliferation and collagen synthesis of HSC- T6 cells stimulated with SEA-MCM were measured using MTT colorimetric assay and ^3H-proline incorporation respectively. Results The proliferation and collagen production of HSC-T6 cells were significantly promoted by SEA-MCM. They were significantly suppressed after being treated with AST（32. 5,65,130 mg · L^-1 ） showing concentration-dependent effect. Conelusion： Astragalosides may inhibit HSC-T6 cells proliferation and collagen production that was driven by SEA activated MCM in vitro.

关 键 词：黄芪总苷 血吸虫可溶性虫卵抗原 肝星状细胞 增殖 胶原合成 

分 类 号：R-332[医药卫生] R284.1