豹皮樟总黄酮对大鼠非酒精性脂肪性肝炎的防治作用  

The protective and therapeutic effects of total flavonoids of litsea coreana level on nonalcoholic steatohepatitis in rats

作　　者：倪鸿昌[1] 李俊[1] 金涌[1] 程文明[1] 彭磊[1] 张骏艳[1] 黄艳[1] 

机构地区：[1]安徽医科大学药学院

出　　处：《中国药理学通报》2006年第5期591-594,共4页Chinese Pharmacological Bulletin

基　　金：安徽省人才开发基金资助项目(No2004Z030);安徽省自然科学基金资助项目(No03043001)

摘　　要：目的研究豹皮樟总黄酮(TFLC)对大鼠非酒精性脂肪性肝炎(NASH)的防治作用。方法SD大鼠48只,随机分为6组(n=8):对照组、模型组、烟酸组及TFLC组(50、100、200 mg.kg-1)。除对照组外,其余各组每天给予脂肪乳剂和相应药物。实验开始后3 d收集72 h的粪便,测定粪便中脂质;于1 wk后断尾取血,测定血清脂质;3 wk后处死动物,测定血清甘油三酯(TG)、总胆固醇(TC)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、丙二醛(MDA)、超氧化物歧化酶(SOD)和肝匀浆TG、TC、MDA、SOD,并行肝脏病理学检查。结果模型组大鼠出现NASH,烟酸组及TFLC组(100、200 mg.kg-1)均能降低血清和肝脏TG,肝脂肪变性程度和炎症均明显减轻;TFLC组(200 mg.kg-1)还能降低血清和肝MDA,提高肝组织SOD活性。结论TFLC可以降低大鼠血和肝组织TG,且能改善NASH大鼠脂肪变,减轻炎症反应。其机制可能是:TFLC抑制TG在肠道的吸收,同时减轻脂质过氧化。Ahn To study the preventive and therapeutic effects of total flavonoids of Litsea Coreana level on nonalcoholic steatohepatitis in rats. Methods 48 SD rats were randomized into control , model , NA, TFLC （50,100,200 mg · kg^-1 ）groups（n =8）. The control group were only fed with a standard diet and the other groups were fed with a standard diet and fat emulsion（ 10 ml · kg^-1 ） ;At the same time,the control and model groups were fed with CMC-Na, the other groups were fed with relative medicine. The stool TG ,TC ,the serum TG,TC, ALT, AST, MDA, SOD and hepatic TG, TC, MDA,SOD were detected dynamically. Results The model group developed NASH after 3 weeks. NA and TFLC（ 100,200 mg · kg^-1） significantly reduced TG of the serum and liver tissue, and ameliorated hepatocellular macrovesicular steatosis. TFLC （200 mg · kg^-1 ） markedly inhibited increased MDA in serum and liver tissue ,and also elevated SOD in liver tissue. Conclusions TFLC markedly reduced the TG level of serum and liver tissue. Liver histopathological examination showed TFLC ameliorated hepatocellular macrovesicular steatosis. The protective and therapeutic effects of TFLC on NASH may be associated with inhibitory effect of fat absorption and lipid peroxidation.

关 键 词：豹皮樟总黄酮 非酒精性脂肪性肝炎 防治作用 大鼠 

分 类 号：R-332[医药卫生] R284.1