葛根素对谷氨酸所致大鼠原代神经细胞损伤的保护作用  

Protective effects of puerarin on rat primary cultured neurons damaged by glutamic acid

作　　者：陈立敏[1] 吕秋军[1] 温利青[1] 陈媛媛[1] 陈振花[1] 谢洁琼[1] 张敏[1] 曹颖林[2] 

机构地区：[1]军事医学科学院放射和辐射医学研究所药理毒理研究室,北京100850 [2]沈阳药科大学,辽宁沈阳110016

出　　处：《中国药理学通报》2006年第5期607-611,共5页Chinese Pharmacological Bulletin

摘　　要：目的从膜脂流动性等观察葛根素对谷氨酸所致大鼠原代神经细胞损伤的保护作用。方法建立大鼠原代神经细胞谷氨酸损伤模型,以1,6-二苯基-1,3,5-己三烯(DPH)为探针,用荧光偏振法测定荧光偏振度(P)值和微粘度(η),研究细胞膜脂流动性的改变;MTT(四甲基偶氮唑盐)法、培养介质乳酸脱氢酶(LDH)活力测定观察葛根素的保护作用。结果葛根素组荧光偏振度和微粘度低于谷氨酸损伤组,与尼莫地平作用相当,且呈现剂量依赖关系。葛根素组原代神经细胞LDH的释放减少,吸光度增加,但葛根素对正常细胞增殖无影响。结论葛根素对谷氨酸所致的大鼠原代神经细胞损伤具有保护作用,其保护作用可能与葛根素改善神经细胞膜脂流动性有关。Aim To study the protective effects of puerarin on the neurons damaged by glutamic acid. Method The rat primary cultured neurons were damaged by glutamic acid, the membrane lipids fluidity was studied with fluorescence polarization （P） and mean microviscosity （η） with DPH as a probe;the protective effects of puerarin on this model were evaluated by lactate dehydrogenase （LDH） efflux assay and colormetric MTT assay. Results The fluorescence polarizating and mean microviscosity of puerarin were lower than glutamic acid model group in a dose-depended manner. Treatment of puerarin resulted in the decrease of LDH release from primary cultured neurons damaged by glutamic acid and increase in the optical density （A） at 540 nm tested by colorimetric MTT assay. No proliferating effects on normal primary cultured neurons were observed. Conclusion The results suggested that puerarin had a protective effect on rat primary cultured neurons damaged by glutamic acid, it might be relative to the improvement of membrane lipids fluidity

关 键 词：葛根素 原代神经细胞 膜脂流动性 荧光偏振度 DPH 

分 类 号：R-332[医药卫生] R282.71