ERK PI3-K在乳腺癌及癌前组织中的表达及相关性  

The Expression and Correlation of ERK2 and PI3-K in Breast Cancer and Premalignant Tissue

作　　者：马萍[1] 李柏林[2] 张英[3] 宋敏[3] 宋继谒[3] 

机构地区：[1]中国医科大学附属第一医院肿瘤研究所第二研究室,沈阳市110001 [2]中国医科大学基础医学院化学教研室 [3]中国医科大学附属第一医院肿瘤研究所病理教研室,沈阳市110001

出　　处：《中国肿瘤临床》2006年第14期805-807,共3页Chinese Journal of Clinical Oncology

基　　金：辽宁省教育厅高等学校科研基金资助(编号:2004D107)

摘　　要：目的:分析乳腺癌、癌前病变及正常组织中ERK和PI3-K的mRNA、蛋白表达及激酶活性,探讨其在乳腺癌发生和发展中的作用和相互关系。方法:应用RT-PCR方法检测mRNA表达;Westernblot检测蛋白表达;激酶活性检测其磷酸转移酶效率。结果:乳腺癌中ERK2mRNA和蛋白表达均增加(100%,37/37);激酶活性增加(75.68%,28/37)。有32例PI3-KmRNA表达增加(86.49%);35例蛋白表达增加(94.59%);25例激酶活性增加(67.57%)。ERK在乳腺癌和癌前病变中均处于活化状态,PI3-K只在乳腺癌中处于高表达。相关性分析表明,乳腺癌中ERK2与PI3-K激酶活性呈负相关。结论:ERK和PI3-K途径在乳腺癌发生过程中均存在异常且两者可能存在某种协调关系。To investigate the mRNA expression level, protein expression level and kinase activity of both ERK and PI3-K in breast cancer, atypical hyperplasia, benign growth.and adjacent normal mammary glands, and to discuss the correlation between them. Methods： RT-PCR, Western blot and kinase activity assay were used to detect the mRNA expression level, protein expression level and kinase activity of ERK and PI3-K, respectively. Results： For ERK, mRNA expression, protein level and kinase activity were 100%, 100% and 75.68% respectively. For PI3-K, mRNA expression, protein level and kinase activity were 86.49%, 94.59% and 67.57%, respectively. ERK was activated during the stages of both initiation and progression, while PI3-K was activated only in the progression stage of breast cancer. There was an inverse correlation between the activity of ERK2 and PI3-K in breast cancer. Conclusion： The results indicate that both ERK2 and PI3-K pathways are involved in breast cancer and may imply coordination between the two pathways in breast tumorigenesis.

关 键 词：P13-K ERK2 乳腺癌 细胞信号转导 

分 类 号：R737.9[医药卫生—肿瘤]