利用马铃薯茎段离体筛选耐盐变异体  

In Vitro Selection of Salt-tolerant Mutant from Potato Stem Explants

作　　者：程智慧[1] 李娟[1] 张国裕[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《园艺学报》2006年第3期635-638,共4页Acta Horticulturae Sinica

基　　金：中德政府间合作项目(GH-01/04);中德农业部科技合作项目

摘　　要：以4个马铃薯品种的茎段为外植体,在含不同浓度NaCl的培养基上直接诱导耐盐愈伤组织,筛选耐盐变异体.结果表明,不同品种的耐盐性有差异,‘东农303’和‘鄂1号’愈伤组织诱导的最大耐盐浓度为0.8%,‘费乌瑞它’为0.6%,‘夏波帝’为0.4%.盐胁迫抑制愈伤组织芽和根的分化,耐盐愈伤组织继代不宜超过8～10次.盆栽和离体鉴定表明,筛选出的耐盐变异体保持了耐盐的特性.The stem explants of four potato cuhivars were used to induce calli and to screen salt-tolerant mutants in the medium containing different levels of NaCl. The results indicated that different cuhivars showed different tolerance levels. To calli induction, the highest salt tolerant level is 0. 8% for cv. Dongnong 303 and E 1,0. 6% for cv. Favorite and 0. 4% for cv. Shepody. Salt inhibited shoot and root differentiation. The salttolerant calli should be subcuhured for not more than 8 - 10 times. Salt tolerant tests in vivo and in vitro revealed that the mutants possessed salt tolerance.

关 键 词：马铃薯 耐盐变异体 茎段外植体 愈伤组织 离体培养 

分 类 号：S532[农业科学—作物学]