变温及GA_3处理对辽东楤木种子解除休眠过程中代谢调控的影响  

Effect of Alternating Temperature and Gibberellin (GA_3) on Metabolic Control of Japanese Aralia (Aralia elata) Seed In Dormancy-Breaking Process

作　　者：宁伟[1] 范文丽[1] 李宏博[2] 刘延吉[2] 葛晓光[1] 

机构地区：[1]沈阳农业大学野菜研发中心,辽宁沈阳110161 [2]沈阳农业大学生物技术学院,辽宁沈阳110161

出　　处：《园艺学报》2006年第3期649-652,共4页Acta Horticulturae Sinica

基　　金：国家高技术研究发展计划项目(2004AA247010);国家‘十五’科技攻关项目(2001BA503B);辽宁省教育厅科技攻关项目(05L341)

摘　　要：研究在变温条件下GA3对辽东楤木种子解除休眠过程和呼吸代谢途径关键酶(磷酸己糖异构酶、6-磷酸葡萄糖脱氢酶、6-磷酸葡萄糖酸脱氢酶、琥珀酸脱氢酶)的活性变化。结果表明:GA3经200mg·L-1的丙酮溶液浸泡种子12h,其α-淀粉酶活性和可溶性糖的含量显著提高,均在第12天达到最大值;从层积开始可溶性蛋白含量就不断降低,在第12天降到最低点;种子在后熟过程中主要以糖酵解和三羧酸循环作为供能形式。在GA3和层积变温处理过程中呼吸代谢途径关键酶的活性在第8天达到最大值,表明磷酸戊糖途径的活化对解除种子休眠起到至关重要的作用。This study investigated the change 6-phosphogluconate dehydrogenase and succinic dehydrogenase and in the contents of metabolic substances in the activity of key enzymes for respiratory metabolic pathway hexosephosphate isomerase, 6-zwischenferment, in the Japanese aralia （Aralia elata） seed dormancybreaking process under alternating temperature. Results showed that, after the seed was soaked in a 200 mg · L^-1 GA3 acetone solution for 12 hours, the α-amylase activity and the soluble sugar content in the seed increased significantly and reached the maximum on the 12th day, whereas the soluble protein content decreased steadily since the initiation of stratification, down to the lowest point on the 12th day. In the after-ripening process, the Japanese aralia seed mainly relied on glycolysis and tricarboxylic acid cycle for its energy supply through oxidation, the activity of key enzymes in phosphopentase pathway reached the maximum on the 8th day, in GA3 and stratification alternating treatment proceess, suggesting that the activation of this pathway also played a vital role in breaking the dormancy of the seed.

关 键 词：辽东楤木 后熟 GA3 层积 代谢调控 

分 类 号：S63[农业科学—蔬菜学]