丁酸钠对食管癌Eca-109细胞增殖和凋亡的作用  

The effects of sodium butyrate on proliferation and apoptosis of esophageal carcinoma Eca-109 cells

作　　者：沈蓉[1] 陶仪声[1] 

机构地区：[1]蚌埠医学院病理学教研室,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2006年第4期336-338,共3页Journal of Bengbu Medical College

基　　金：安徽省教育厅自然科学研究资助项目(2002kj209)

摘　　要：目的：观察丁酸钠对食管癌Eca-109细胞增殖和凋亡的作用。方法：以1、2、5、5、5mmol／L丁酸钠处理Eca-109细胞，倒置显微镜观察细胞形态变化，透射电镜观察细胞凋亡的形态，四甲基噻唑氮蓝法检测抑制细胞增殖率，TUNEL法检测细胞凋亡率。结果：Eca-109细胞经丁酸钠处理后：光镜下观察细胞变小、变圆，部分从培养瓶壁上脱落下来；电镜下细胞核体积缩小，染色质浓缩聚集于核膜处；细胞增殖受到明显抑制，1、2．5、5mmol／L丁酸钠处理24h、48h和72h后，细胞生长抑制率分别是13．38％、34．15％、39．02％，14．04％、33．33％、46．49％和31．11％却．74％．48．15％，与低浓度组比较差异有统计学意义（P〈0．05-P〈0．01）；2．5mmon／L丁酸钠处理24h组与阴性对照组细胞凋亡率分别是5．5％和1．6％，差异有统计学意义（P〈0．01）。结论：丁酸钠可抑制食管癌Eca-109细胞增殖、促进凋亡，且呈浓度和时间依赖性。Objective：To investigate the effects of sodium butyrate（NaB） on proliferation and apoptosis of esophageal carcinoma Eea- 109 cells. Methods： Eca-109 cells was treated with NaB in concentration of 1,2.5,5 mmol/L. Morphological changes of Eca-109 cells were observed by inverted microscope. The morphology of apoptotic cells was investigated by transmission electronic microscope. The inhibition rate of ceil＇growth was assessed by MTI＂ assay. TUNEL assay was used for estimating apoptotic rate. Results：Treatment of Eca-109 cells with NaB resulted in significant inhibition of cell growth in time-dependent and dose-dependent manners. Cells became small and round ,many of them fell off from the wall under light microscopy. NaB could reduce the solidity of nuclei ,make the chromatin condense and get together into karyotheca. NaB inhibited the growth rate of cells obvioursly, the growth rates of cells treated with NaB in concertrations of 1,2.5,5 mmol/L for 24 h, 48 h and 72 h were 13.38%, 34. 15% , 39.02% ; 14.04%, 33.33% , 46. 49% ; and 31.11% ,40.74% ,48.15 %, respectively （ P 〈 0.05 - P 〈 0.01 ）. The rates of cell apoptosis in the group treated with 2.5 mmol/L NaB for 24 h and control group were 5.5% and 1.6%, respectively （P 〈 0.05 ）. Conclnsions： Sodium butyrate could inhibit the proliferation and promote the apoptosis in esophageal carcinoma Eca-109 cells in time-dependent and dose-dependent.

关 键 词：食管肿瘤 丁酸钠 ECA-109细胞 细胞增殖 细胞凋亡 

分 类 号：R735.1[医药卫生—肿瘤]