Apoptin对C6胶质瘤细胞凋亡的诱导作用  

Induction of apoptin on apoptosis of C6 glioma cells

作　　者：朱战鹏[1] 邱吉庆[1] 连海[2] 郭云宝[3] 罗毅男[1] 

机构地区：[1]吉林大学第一医院神经外科,吉林长春130021 [2]中国人民解放军军事医学科学院基因工程研究室,吉林长春130062 [3]吉林大学第一医院心外科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2006年第4期579-581,F0002,共4页Journal of Jilin University：Medicine Edition

基　　金：国家973计划资助课题(G1999011902)

摘　　要：目的:探索apoptin对C6胶质瘤细胞凋亡的诱导作用。方法:构建荷C6胶质瘤Wistar大鼠的肿瘤模型,随机分为荷瘤对照组、化疗对照组和pvVP3组,给予不同的药物,通过超微结构分析、TUNEL法检测肿瘤细胞凋亡率,观察apoptin诱导C6胶质瘤细胞的凋亡情况。结果:pvVP3组肿瘤细胞异染色质边集,细胞核固缩,凋亡小体形成,而荷瘤对照组则未见明显凋亡改变。TUNEL法检测肿瘤细胞pvVP3组凋亡率为51.11%,明显高于荷瘤对照组(28.96%)(P<0.01)。结论:apoptin能够诱导C6胶质瘤细胞凋亡。Objective To explore the induction of apoptin on apoptosis in C6 glioma cells. Methods After having successfully cultivated C6 glioma cells and had an inoculation in Wistar rats, the animal models of Wistar rats bearing C6 glioma were set up. The rats were randomly divided into three groups： tumor control, chemotherapy and pvVP3 groups. Different treatment methods were performed in different groups, The ultrastructural changes and the apoptotic rates were detected with TUNEL. Results Typical changes of apoptosis in pvVP3 group were found through ultrastructural observation, while they could not be found in tumor control group, The apoptotic rate in pvVP3 group （51.11%） was higher than that in control group （28. 96%） （P〈0. 01）. Conclusion Apoptin can induce apoptosis of C6 glioma cells.

关 键 词：APOPTIN 鸡贫血病毒 神经胶质瘤 细胞凋亡 

分 类 号：Q255[生物学—细胞生物学] R739.41[医药卫生—肿瘤]