肝癌患者淋巴细胞染色体脆性部位与原癌基因的相关性  

Correlation between chromosome fragile site and proto-oncogene in patients with hepatocellular carcinoma

作　　者：曹岩[1] 刘红[2] 刘永红[3] 

机构地区：[1]吉林大学第二医院中心实验室,吉林长春130041 [2]吉林大学口腔医院修复科,吉林长春130041 [3]吉林省肿瘤医院电诊室,吉林长春130012

出　　处：《吉林大学学报（医学版）》2006年第4期662-664,共3页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅资助课题(970565-3)

摘　　要：目的:探讨肝癌患者外周血淋巴细胞染色体脆性部位与原癌基因表达率的相关性。方法:采用低叶酸、低小牛血清、较高pH值和G显带培养方法,常规外周血染色体制片。对20例住院肝癌患者(实验组)和18例献血队员(对照组)的外周血淋巴细胞染色体脆性部位(Fra)及染色体畸变(CAR)进行分析。结果:实验组检出44种脆性部位,主要分布于A、B、C组染色体,其表达率为9.90%,与对照组(0.67%)比较,差异有显著性(P<0.01)。脆性部位中有11种与原癌基因重叠,重叠率为25%。结论:检出染色体的脆性部位可能与肝癌的发生、发展有关。Objective To explore the correlation between chromosome fragile site in peripheral blood lymphocytes and proto-oncogene expression rate in patients with hepatocellular carcinoma. Methods The peripheral blood lymphocyte chromosome preparation was made routinely. The cells were cultivated with low concentrations of calf serum and folic acid, higher pH medium and G-banding method. The fragile sites in 20 patients with hepatocellular carcinoma （experimental group） and 18 healthy subjects （control group） were exactly located and analyzed. Results The fragile sites in experimental group were determined as 44 species mainly distributed on the chromosomes of A, B, C group. The expression rate of the fragite site in experimental group （9.90%） was significantly higher than that in control group （0. 67%） （P〈0.01）. 11 among the fragite sites overlapped the protooncogene sites and the superposition frequency was 25 %. Conclusion The chromosome fragile sites detected in this paper may be related to the carcinogenesis and development of liver cancer.

关 键 词：肝肿瘤 染色体脆性 原癌基因 淋巴细胞 

分 类 号：R735.7[医药卫生—肿瘤] Q813.11[医药卫生—临床医学]