FHIT基因在子宫内膜癌中的表达及意义  

Expression of fragile histidine triad gene in endometrial carcinoma and its significance

作　　者：华莹[1] 谷丽萍[2] 于伟[2] 张首杰[2] 

机构地区：[1]温州医学院第二附属医院妇产科,浙江温州325000 [2]吉林大学第二医院妇产科,吉林长春130041

出　　处：《吉林大学学报（医学版）》2006年第4期669-671,F0003,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科技发展项目资助课题(20030538-1)

摘　　要：目的：探讨子宫内膜癌中脆性组氨酸三联体（FHIT）基因的表达及其与临床病理指标的可能关系。方法：采用免疫组化S-P法检测36例子宫内膜癌、17例子宫内膜上皮内瘤变、14例正常子宫内膜组织标本中FHIT的表达。结果：子宫内膜癌、子宫内膜上皮内瘤变及正常子宫内膜组织中FHIT的阳性表达率分别为50％（18／36）、88．26％（15／17）和92．86％（13／14）；正常子宫内膜与子宫内膜瘤变组织中FHIT阳性表达率比较，差异无显著性（P〉0．05）；正常子宫内膜、子宫内膜瘤变分别与子宫内膜癌组织FHIT阳性表达率比较，差异均有显著性（P〈0．01）。FHIT阳性表达在子宫内膜癌组织学分级的分布为Ⅰ级57．89％，Ⅱ级50％，Ⅲ级28．57％，各分级组间比较差异无显著性（P〉0．05）。FHIT阳性表达在子宫内膜癌手术病理分期的分布为Ⅰ期64％、Ⅱ期20％、Ⅲ～Ⅳ期16．67％，三组间比较差异无显著性（P〉0．05）。子宫内膜癌有淋巴结转移组与无淋巴结转移组FHIT的阳性表达率分别为16．67％（1／6）和66．67％（14／21），差异无显著性（P〉0．05）。子宫内膜癌中浅肌层侵润与深肌层侵润FHIT阳性表达率分别为65％和31．25％，两者比较差异无显著性（P〉0．05）。结论：子宫内膜组织中FHIT基因表达降低可能与子宫内膜癌的发生有关，但与手术病理分期、肌层侵润深度及淋巴结转移无关，该基因在子宫内膜癌组织中表达降低进一步揭示了子宫内膜癌的发病机制。Objective To investigate the expression of fragile histidine triad （FHIT） gene and the possible relationship between FHIT expression and clinicopathological indices in endometrial carcinoma. Methods FHIT protein expressions were detected by immunohistochemical method in 36 cases of endometrial carcinomas （EC）, 17 endometrial intraepithelial neoplasia （EIN） and 14 normal endometrial tissues （NET）. Results The positive rates of FHIT in EC, EIN and NET were 50% （18/18）, 64. 71% （11/17） and 92.86% （13/14）, respectively, and there was no significant difference between EIN and NET （P〉0. 05）, and there was significant difference between EC and EIN or between EC and ENT （P〈0. 01） ; in EC, the expressions of FHIT in histological grade Ⅰ- Ⅲ were 57.89% （11/19） , 50% （5/10） and 28.57% （2/7）, respectively, there was no significant difference between the three groups （P〉0. 05） ; the expression of FHIT in surgical pathologic Staging Ⅰ,Ⅱ, Ⅲ-Ⅳ were 64% （16/25）, 20% （1/5） and 16.67% （1/6）, respectively, there was no significant difference between the three groups （P〉0.05）; the expression of FHIT in lymph node metastatic group was 16.67% （1/6）, and in nonmetastatic group was 66.7% （14/21）, there was no significant difference between the two groups （P〉0. 05）; the expression of FHIT in superficial myometrial invasion group and deep group was 65% （13/20） and 31.25% （5/ 16）, there was no significant difference between the two groups （P〉0.05）. Conclusion The reduced expression of FHIT may be associated with endometrium canceration and may be not associated with surgical pathologic staging, lymph node metastatic and depth of myometrial invasion in endometrial carcinoma. The reduced expression of FHIT indicates the pathogenesis of endometrial carcinoma.

关 键 词：子宫内膜肿瘤 脆性组氨酸三联体 基因表达 免疫组织化学 

分 类 号：Q786[生物学—分子生物学] R737.33[医药卫生—肿瘤]