褐藻多糖高产菌株的构建  

Construction of Alginate High-Yielding Strain of Pseudomonas sp.QDA

作　　者：沈洪波[1] 韩峰[1] 林育姿[1] 王传宁[1] 于文功[1] 

机构地区：[1]中国海洋大学教育部海洋药物重点实验室,海洋药物与食品研究所,山东青岛266003

出　　处：《中国海洋大学学报（自然科学版）》2006年第4期631-634,626,共5页Periodical of Ocean University of China

基　　金：国家自然科学基金面上项目(30371683)资助

摘　　要：Pseudomonassp.QDA是从海水中发现的产生褐藻多糖的细菌,无致病性。为提高QDA菌株褐藻多糖的产量,本文利用PCR克隆了粘性菌株P.aeruginosaFRD1的algT基因,连接入质粒pMF36-Kan,构建了重组表达载体pMF36-Kan-algT。利用三亲接合法将pMF36-Kan-algT转入菌株QDA中。糖醛酸含量测定结果表明,algT基因过量表达菌株的褐藻多糖产量比出发菌株QDA平均提高了3.3倍,其中菌株A25提高了4.5倍。通过培养条件的进一步优化,A25菌株的褐藻多糖产量与菌株QDA相比提高到8.18倍。Pseudomonas sp. QDA, which was isolated from seawater, has the ability of yielding alginate. In order to enhance the alginate production of Pseudomonas sp. QDA, algT gene of mucoid strain Pseudomonas aeruginosa FRD1 was cloned by PCR and ligated into plasmid pMF36-Kan. The resulting plasmid pMF36- Kan-algT was transferred into ODA by triparental mating. The average alginate production of algT over-expressed clones are 3.3 folds higher than that of wild strain QDA, and clone A25 has the highest yield and is about 4.5 folds higher than that of wild strain QDA. After further optimization of the culture condition, the alginate production of A25 is 7.18 folds higher than that of wild strain QDA.

关 键 词：假单胞菌 褐藻多糖 algT基因 过量表达 

分 类 号：Q539[生物学—生物化学]