非霍奇金淋巴瘤细胞图像分析研究  

Image cytometry DNA analysis in NHL

作　　者：刘勇[1] 苏祖兰[1] 龙捷[2] 金亦[1] 冯智英[1] 

机构地区：[1]中山大学附属第三医院病理科,广州510630 [2]广州医学院病理教研室,广州510000

出　　处：《中国体视学与图像分析》2006年第2期117-121,共5页Chinese Journal of Stereology and Image Analysis

基　　金：国家自然科学基金资助项目(No.39870328);广东省科技重点攻关基金(No.C30904);广东省卫生厅科技基金(No.2001207)

摘　　要：目的 应用细胞图像分析术（ICM—DNA）对大宗病例非霍奇金淋巴瘤（NHL）进行DNA参数的检测，寻找对NHL的诊断、鏊别诊断和预后有指导意义的参考指标。方法 对210例NHL（WH02001新分类）病例及30例良性增生淋巴组织（RH）行改良的Feulgen染色并作ICM-DNA检测。结果 NHL平均DNA含量为7．01～10．77Pg；SPF为10．97％～30．87％，异倍体率7．76％～17．76％，均高于对照组（P≤0．01）；NHL二倍体百分含量为16．03％～70．90％，低于对照组（P≤0．01）；NHL侵袭组、高侵袭组的平均DNA含量、SPF、异倍体率均高于惰性组（p≤0，01）；FL—Ⅱ、Ⅲ级的平均DNA含量、SPF、异倍体率均高于FL-Ⅰ级（p≤0．01）。结论 ICM—DNA检测中，平均DNA含量、SPF、异倍体率、二倍体百分含量对良恶淋巴组织增生的鉴别诊断、NHL惰性或侵袭性预后判断均为有意义的参考指标；但对侵袭性高低无提示，FL是相对异质化群体，不能简单归为惰性。Objective To investigate the dignositic and prognositic significance of DNA parameters by DNA image cytometry （ICM-DNA） analysis in the non-Hodgkin＇ s lymphoma （NHL）. Methods ICM- DNA was performed on 210 NHL and 30 RH specimens prepared by Feulgen stain. DNA content, Diploid fraction, Aneuploid fraction, and S-phase fraction （SPF） were computed. Result DNA content （7.01～ 10.77 pg）, SPF（ 10.97 ～ 30.8）, and Aneuploid fraction （7.76% ～ 17.76% ） in NHL was higher than in RH（p≤0.01 ） ; but Diploid fraction （16.03% ～70.90% ）.in NHL was lower. There is significant difference between indolent and aggressive, indolent and highly aggressive, FL-Ⅰ and FL-Ⅱ, FL-Ⅰ and FL-Ⅲ （P≤0.01）, but nonsignificant difference between aggressive and highly aggressive, FL-Ⅱ and FL-Ⅲ in all parameters investigated （P 〉 0.05 ）. Conclusion DNA content, Diploid fraction, Aneuploid fraction, and SPF can be useful in distinguishing between malignant and benign lymphoid hyperplasias, indolent and aggressive/highly aggressive, but none of them could differentiates between aggressive and highly aggressive; FL should not be simply taken as indolent NHL.

关 键 词：非霍奇金淋巴瘤 ICM—DNA 

分 类 号：R733.1[医药卫生—肿瘤]