联合表位肽诱导HLA-A2^+人PBMC产生抗原特异性CTL及杀伤活性研究  

Polypeptides based on CTL of MAGE-3 and MAGE-n antigens induce HLA-A2^+ human PBMCs to produce CTL-mediated cytotoxicity

作　　者：张秀敏[1] 隋延仿[1] 司少艳[1] 胡沛臻[1] 黄杨[1] 葛伟[1] 李侠[1] 马斌[1] 

机构地区：[1]第四军医大学基础部病理学教研室,陕西西安710033

出　　处：《现代肿瘤医学》2006年第8期913-916,共4页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(30271464)

摘　　要：目的:探讨MAGE-3,MAGE-n抗原表位体外联合诱导的CTL,并研究其特异性杀伤活性。方法:候选抗原表位以固相多肽合成技术合成,并用HPLC进行纯化,质谱法(MS)鉴定,以流式细胞仪筛选HLA-A2+人外周血PBMC,T2细胞负载抗原肽反复刺激活化诱导抗原特异性CTL,LDH检测其杀伤活性。结果:联合表位肽体外刺激人PBMC,能够较强地诱导抗原特异性CTL并产生特异性杀伤。结论:MAGE-3与MAGE-n的HLA-A2限制性CTL表位肽的联合应用能够产生较强的体外抗肿瘤免疫反应。Objective ：To explore peptide-specific CTLs response and the immunological activity of the HLA- A2-restricted CTL candidate epitopes derived from tumor antigen MAGE-3 and MAGE-n in vitro. Methods： The MAGE-3 and MAGE-n candidate HLA-A2 restricted CTL epitopes were synthesized with solid phase strategies, purified with reverse phase HPLC and identified with mass spectrometry. Peptide-specific CTLs were induced from the peripheral blood mononuclear cells （PBMC） of HLA-A2 positive healthy donors by multiple stimulations with peptide-pulsed T2 cells. The capability of CTLs recognizing and lysing HLA-A2^＋ MAGE- 3^＋ and MAGE- n ＋ tumor cells was detected with lacatate dehydrogenase （LDH） release assay. Results： The results demonstrated the combination of MAGE -3 and MAGE-n epitope tumor-specific CTL responsed in vitro specifically and effectively. Conclusion： Combination of two epitopes could elicit stronger MAGE-specific antitumor immunity.

关 键 词：肿瘤抗原 MAGE-3 MAGE-N 细胞毒性T淋巴细胞 表位 

分 类 号：R73-36[医药卫生—肿瘤]