流式细胞术检测肺癌患者外周血微转移的探讨  

Detecting micromatastasis in the peripheral blood of patients with lung cancer by flow cytometry

作　　者：牛中喜[1] 郭金成[2] 汪良骏[1] 朱金如[1] 李树军[1] 

机构地区：[1]武警总医院胸外科,北京100039 [2]河南焦作市第二人民医院胸外科,河南焦作454000

出　　处：《现代肿瘤医学》2006年第8期950-951,共2页Journal of Modern Oncology

基　　金：武警总部基金资助(项目编号:WZ200316)

摘　　要：目的:应用流式细胞术检测肺癌患者外周血中的微转移。方法:外周血经F icoll梯度离心分离单核细胞后,用粘蛋白1(Muc in1,MUC1)单克隆抗体染色,应用流式细胞仪检测。结果:检测了96例肺癌患者外周血,发现29例患者外周血中有微转移。32例肺良性病变患者有1例患者外周血中有微转移。肺癌患者阳性率与患者病理分期、分化程度和细胞组织学类型密切相关(P<0.05)。结论:应用流式细胞术检测非小细胞肺癌患者外周血中的微转移,有助于判断肺癌转移倾向和预后。Objective： To detect micrometastasis in the peripheral blood of non small cell lung cancer（NSCLC） patients by flow cytometry（FCM）. Methods： The monotype fraction in peripheral blood was isolated by FicollHypaque gradient centrifugation. The ceils obtained were labeled with antibodies against MUC1, then analyzed by FCM. Results： Twenty nine cases out of 96 patients with NSCLC were found to have micromatnstnsis in the peripheral blood . One case was found to have micromatnstnsis in 32 lung benign patients. There was distinct correlation between detective rate of micromatnstnsis and pathological classification, cell differentiation of the cancer and P-TNM stage of the disease. （P 〈0.05 ）. Conclusion： Examination of he valuable in predicting metastasis and prognosis of NSCLC.

关 键 词：非小细胞肺癌 流式细胞术 微转移 外周血 

分 类 号：R734.2[医药卫生—肿瘤]