高效液相色谱法测定虫草酒中的虫草素  

Determination of 3′-Deoxyadenosine in Cordyceps sinensis Wine with High Performance Liquid Chromatography

作　　者：张永康[1] 胡江宇[1] 欧阳辉[2] 银永忠[2] 赵建文[2] 

机构地区：[1]吉首大学生物资源与环境科学学院,湖南吉首416000 [2]吉首大学化学化工学院,湖南吉首416000

出　　处：《食品科学》2006年第7期199-201,共3页Food Science

基　　金：湖南省星火计划项目(2000湘科星字第021号)

摘　　要：采用高效液相色谱法建立了虫草酒中虫草素含量的测定方法,探讨了色谱柱类型、流动相组成、柱温以及检测波长对分析结果的影响。研究表明:当色谱柱为WatersSymmetryC18柱(150mm×4.6mmi.d.,5μm);流动相为乙腈-水(体积比为10:90),流速为1ml/min;检测波长258nm;柱温40℃;进样量5μl时分离、检测效果最佳,此法操作简便,样品只需用0.45μm微孔滤膜过滤处理,线性范围宽、重现性好、精密度高,效果令人满意,对于虫草酒的生产和质量检测都具有现实的指导意义。A method to determine 3＇ -deoxyadenosine in Cordyceps sinensis wine has been established with high performance liquid chromatography. The effects of several factors on analysis effect were studied, such as type of chromatogram column, component of mobile phase, column temperature, detective wave length and so on. The studies indicated that the effects of separation and determination are optimum when determination parameters are as follows： analytic column： Waters Symmetry C18（150mm × 4.6mm i.d., 5μm）; mobile phase： acetonitrile-ultrapure water（10 ：90）; flow rate： 1ml/min; detective wave length： 258nm; column temperature： 40℃ and sample volume： 5μl. The method to deal with sample is rather simple as only necessary to be filtrated with 0.45μm filter film. The method is feasible, accurate and satisfactory. It is simple to operate and suitable for the quality control of cordyceps sinensis wine.

关 键 词：高效液相色谱 虫草酒 虫草素 测定 

分 类 号：O652.63[理学—分析化学]