罗汉果中主要皂甙成分的高效液相分析研究  

Study on Analysis of Main Glycosides from Siraitia grosvenorii Fruits with High-performance Liquid Chromatography

作　　者：张云竹[1] 戚向阳[1] 陈维军[1] 张俐勤[2] 

机构地区：[1]华中农业大学食品科技学院,湖北武汉430070 [2]嘉兴学院,浙江嘉兴314000

出　　处：《食品科学》2006年第7期224-227,共4页Food Science

摘　　要：目的:探讨罗汉果皂甙的HPLC分析法,并对罗汉果中主要皂甙成分罗汉果皂甙V、11-O-罗汉果皂甙V、罗汉果皂甙IV以及赛门甙I的含量进行分析。方法:以C18柱为色谱柱,采用反相高效液相法分离分析罗汉果皂甙。结果:建立了罗汉果皂甙的HPLC分析条件,其色谱条件为:流动相,23%的乙腈水溶液;流速1ml/min;检测波长,210nm;柱温,室温;灵敏度,0.04AUFS。采用上述的液相分析方法分析四种罗汉果皂甙的含量,发现该方法简便、快速、准确、线性范围在0.5～13.75μg之间,且线性关系良好(R2≥0.99),加标回收率高(94.46%～105.05%),精密度良好(RSD=1.40%～3.31%)。结论:建立的HPLC方法可用于分析罗汉果及其制品中的罗汉果皂甙含量。Objective： To study the use of HPLC to assay the content of mogroside Ⅴ, 11-oxo-mogroside, mogroside Ⅳ and Siamenoside Ⅰ in Siraitia grosvenorii fruits. Methods： The RP-HPLC was used to determine the content of mogrosides. The analysis was carried on C18 column. Results： The conditions to establish the HPLC method for determination of mogrosides were： eluting solvent, acetonitrile-water, 23：77 （Ⅴ/Ⅴ）; flowrate, 1.0ml/min; detection, 210nm; temperature, ambient and sensitivity setting, 0.04AUFS. The method is simple, easy and of good linear range （0.5 ～13.75μg） with the correlationship over 0.99. The recovery is 94.46%～105.05%, and the RSD 1.40%～3.31%. Conclusion： The HPLC method can be used to determine the content of mogrosides in Siraitia grosvenorii and its products.

关 键 词：高效液相色谱法 罗汉果皂甙 罗汉果 

分 类 号：Q949.782[生物学—植物学]