溃疡性结肠炎肠黏膜组织CD8、Fas／FasL和Bcl-2／Bax的表达及关系  

Distribution of CD8 T cells and expression of Fas/FasL and Bcl-2/Bax in ulcerative colitis

作　　者：张姮[1] 夏冰[2] 杨桂芳[3] 李瑾[4] 

机构地区：[1]武汉市中心医院消化内科,湖北省武汉市430014 [2]武汉大学中南医院内科与病理科,湖北省武汉市430071 [3]武汉大学医学院消化系病研究中心,湖北省武汉市430071 [4]武汉大学医学院过敏与免疫相关疾病研究中心,湖北省武汉市430071

出　　处：《世界华人消化杂志》2006年第18期1795-1798,共4页World Chinese Journal of Digestology

摘　　要：目的:研究溃疡性结肠炎(UC)肠黏膜CD8 T细胞Fas／FasL、Bcl-2／Bax蛋白表达以及相互关系,探讨细胞凋亡机制在UC发病中的作用．方法:采用免疫组化SP法检测60例UC肠黏膜组织以及60例正常肠黏膜组织CD8,Fas／FasL, Bcl-2／Bax蛋白表达．结果:CD8阳性细胞在上皮间的浸润在UC 组为52％,正常组为78％,两组相比差异显著 (P<0．01);急性期较缓解期也显著减少(20％ vs 74％,P<0．01)．CD8在UC患者急性期黏膜固有层的表达为80％,高于缓解期的34％(P= 0．0006)．Fas在UC上皮中表达62％,正常组织 30％,两组相比差异显著(P<0．01);急性期高于缓解期(84％ vs 45％,P<0．01)．FasL在固有膜炎性细胞中的表达UC组为62％,正常组7％, 两组相比差异显著(P<0．00;急性期(88％)高于缓解期(43％),而且CD8与FasL在黏膜固有层炎性细胞的表达呈正相关(X2=7．3,P<0．01)． Bcl-2／Bax在UC肠黏膜上皮的表达率与正常组相近,差异无显著性．结论:UC肠黏膜组织Fas／FasL表达增强, Bcl-2／Bax表达无明显变化,CD8细胞与UC急性期Fas／FasL表达相关．AIM： To investigate the expression of Fas/FasL and Bcl-2/Bax and distribution of CD8 T cells as well as their correlations in ulcerative colitis （UC）, METHODS： Immunohistochemistry was used to detect the expression of CD8, Bcl-2/Bax and Fas/FasL in intestinal mucosal tissues from UC （n = 60） and normal controls （n = 60）, RESULTS： The positive rate of CD8 was significantly higher in the epithelia of UC tissues than that in the controls （52% vs 78%, P 〈 0.01）, and rate in the active UC was also significantly lower than that in the remissive UC （20% vs 74%, P 〈 0,01）. The positive rate of CD8 in the lamina propria tissues of UC at active stage was markedly higher than that at rernissive stage （80% vs 34%,P = 0.0006）. The expression of Fas was remarkably higher in the epithelia of UC tissues than that in the controls （62% vs 30%, P 〈 0.01）, and its expression at active UC was also dramatically higher than that at remissive stage （84% vs 45%, P 〈 0.01）. The expression of FasL was significantly increased in the inflammatory cells from the lamina propria of UC tissues as compared with that from normal mucosa （62% vs 7%, P 〈 0.01）, and it was also a significant different between the active and remissive stage （88% vs 43%, P 〈 0.01）. Furthermore, there was a correlation between the expression CD8 and FasL in the inflammatory cells from the lamina propria （χ^2 = 7.3, P 〈 0.01）. The expression of Bcl-2/Bax was not different between UC and normal mucosa （P 〉 0.05）. CONCLUSION： The expression of Fas/FasL is up-regulated in UC, but the expression of Bcl-2/ Bax is not obviously changed. CD8 T cells play important roles in the development of UC and they are closely related with Fas/FasL system.

关 键 词：CD8细胞 FAS/FASL BCL-2/BAX 溃疡性结 肠炎 

分 类 号：R574.62[医药卫生—消化系统]