缺氧促进猪肺动脉内皮细胞sis／PDGF－B链基因的表达  

Hypoxia induced the expression of the platelet一derived growth factor一Bchain/c一sis gene in porcine pulmonary artery endothelial cells

作　　者：李丰[1] 董艳彬[1] 车东媛[1] 张宏权 

机构地区：[1]同济医科大学病理教研室,锦州医学院病理教研室,中国医学科学院心血管病研究所,军事医学科学基础医学研究所

出　　处：《中国病理生理杂志》1996年第6期562-565,共4页Chinese Journal of Pathophysiology

基　　金：国家"八五"科技攻关课题经费

摘　　要：应用Ｎｏｒｔｈｅｒｎｂｌｏｔ和Ｓｌｏｔ一ｂｌｏｔ杂交技术研究了缺氧对猪肺动脉内皮细胞血小板源性生长因子（ＰＤＧＦ）基因表达的影响。结果表明，缺氧能明显增加肺动脉内皮细胞ＰＤＧＦ一Ｂ链ｍＲＮＡ的表达，而ＰＤＧＦ一Ａ链ｍＲＮＡ表达无明显影响。提示缺氧可能是体内刺激肺动脉内皮细胞ｓｉｓ／ＰＤＧＦ一Ｂ链基因表达的因素之一，通过控制肺动脉平滑肌细胞增生和收缩，在肺动脉高压血管改建中可能具有重要作用。The effect of hypoxia on the expression of the platelet-derived growth factor(PDGF) gene in cultured pulmonary artery endothelial eells (PAEC) was studied byusing Northern blot and Slot blot hybridization technique.The result showed that hypoxia(3%O2)significantly increased PDGFB chain mRNA expression in PAEC,whereas level ofPDGFA chain mRNA was not much affected in comparison with the control(21%O2).Itsuggested that hypoxia might be one stimulus for expression of PDGFB chain in PAEC invivo and played an important role by inducing proliferation and contraction of thepulmonary arterv smooth muscle eells.

关 键 词：缺氧 内皮 肺动脉 血小板 衍化生长因子 

分 类 号：R364.4[医药卫生—病理学] R594.302[医药卫生—基础医学]