SMMC-7721肝癌细胞膜上β_2糖蛋白Ⅰ受体的表达  

Expression of beta-2glycoprotein I receptor on membranes of hepatoma cell line SMMC-7721

作　　者：高沿航[1] 高普均[1] 王丹[1] 时阳[1] 李玉琴[1] 朴云峰[1] 杨翰仪[2] 

机构地区：[1]吉林大学第一医院消化内科,吉林省长春市130021 [2]吉林大学基础医学院生物化学教研室,吉林省长春市130021

出　　处：《世界华人消化杂志》2006年第17期1731-1734,共4页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No 30572106~~

摘　　要：目的:研究β2糖蛋白I(β22GPI)与 SMMC-7721肝细胞膜的相互作用过程,探讨乙型肝炎病毒(HBV)嗜肝性的发生机制．方法:采用荧光显微镜及流式细胞仪分析的方法．检测SMMC-7721、HL-60及SGC-7901 三种细胞膜与FITC-β2GP I相结合的情况以及二者的结合特性．结果:仅SMMC-7721细胞膜表面附有大量荧光,其余2种细胞无此现象．加FITC-β2GP I 20 μL时,SMMC-772 1与FITC-β2GP I 的结合率为19％,明显高于FITC-β2GP I与 HL-60的结合率(1．7％)及与SGC-7901的结合率(1．9％)(P<0．01)．加FITC-β2GP I 50μL 时,SMMC-7721与FITC-β2GP I的结合率为 20．8％,与20 μL的结合率无差异(P>0．05),说明SMMC-7721与FITC-β2GP I的结合率不随 FITC-β2GP I量的增加而增高．结论:在SMMC-7721肝细胞膜表面存在能与人β2GP I特异结合的蛋白．AIM： To further study the interaction between beta-2-glycoprotein Ⅰ （β2GPI） and the membrane of hepatoma SMMC-7721 cells, and to explore the mechanism of hepatitis B virus （HBV） infection. ETHODS： Fluorescence microscopy and fluorescence activated cell sorter （FACS） was used to observe the interaction of β2GP Ⅰ with the hepatoma cell line SMMC-7721, gastric carcinoma cell line SGC-7901 and lymphoma cell line HL-60. RESULTS： Fluorescence microscopy revealed specific binding of FITC-β2GP I to SMMC-7721 cells, but neither to HL-60 nor SGC-7901 cells. FACS analysis demonstrated that the binding rate of FITC-β2GP Ⅰ （20 μL） to SMMC-7721 cellswas significantly higher than that in HL-60 or SGC-7901 cells （19% vs 1.7%, 1.9%, both P 〈 0.01）. When 50 μL FITC-β2GP Ⅰ was used, the binding rate of FITC-β2GP Ⅰ to SMMC-7721 cells was 20.8%, which was not significantly higher than 19%. The binding rate to SMMC-7721 cells did not increase with the increasing amounts of FITC-β2GP Ⅰ. CONCLUSION： There exists the specific β2GP Ⅰ binding protein in the membrane of SMMC-7721 cells.

关 键 词：肝癌 SMMC-7721经胞系 β2糖蛋白Ⅰ 乙型肝炎病毒 受体 流式细胞术 

分 类 号：R735.7[医药卫生—肿瘤] R512.62[医药卫生—临床医学]