兔VX2肝癌模型制作的完善及综合影像学评价  

Consummation in making rabbit model bearing VX2 liver tumor and multiple imaging evaluation

作　　者：陈晟[1] 邓钢[1] 牛焕章[1] 何仕诚[1] 方文[1] 郭金和[1] 马占龙[1] 汪盛齐[1] 李国昭[1] 滕皋军[1] 

机构地区：[1]东南大学附属中大医院放射科,江苏南京210009

出　　处：《中国介入影像与治疗学》2006年第4期297-301,共5页Chinese Journal of Interventional Imaging and Therapy

基　　金：国家自然科学基金资助项目(30470507);江苏省社会发展基金资助项目(7790003034)

摘　　要：目的进一步完善兔VX2肝癌模型的制作,比较兔VX2肝癌的CT、MRI、DWI及DSA影像表现,评价各影像检查对瘤体显示的优越性。方法实验动物为新西兰大白兔。模型制作前期实验采用开腹VX2瘤粒包埋法接种,后期采用开腹VX2瘤粒悬浮液针头注入法,均接种于兔肝左叶。接种两周后行CT、MRI及DWI成像,随后行经股动脉肝动脉超选择DSA。结果前期实验瘤块种植成功率66.7%(8/12),后期实验种植成功率100%(24/24)。CT平扫肿瘤为略低密度病灶,强化不明显,境界清晰;MR T1WI呈较低信号影,EPI序列T2WI呈略高信号影;DWI成像见高亮信号影,显示清晰;DSA造影可见肝左叶孤立的瘤体由增粗的动脉供血,肿瘤血管较丰富。结论兔VX2肝癌模型是介入治疗实验研究极为理想的动物模型。VX2瘤粒悬浮液针头注入法较瘤粒包埋法成功率更高。CT、MR平扫、EPI序列及DWI成像对于肿瘤的影像评价互有优势,EPI及DWI成像可更敏感地显示肿瘤,DSA可清晰地显示肿瘤的供血。Objective To consummate the facture of rabbit model bearing VX2 liver tumor ulteriorly, compare the image performance of CT, MRI, DWI and DSA in rabbit bearing VX2 liver tumor and evaluate the superiority of each image check-up for the tumor show. Methods New Zealand white rabbits were embeded with VX2 tumor particles in the left lobe of the liver in the former experiments while which were injected into the lobe in the latter experiments. CT scanning and MR imaging including DWI sequence was performed in all of the rabbits 2 weeks later, then the super-selected transcatheter hepatic arterial DSA were performed. Results The successful rate of implantation was 66. 7% （8/12） in formal experiments and 100.0% （24/24） in latter experiments. The tumor was demonstrated as low density mass on plain CT, unconspicuous enhanced on arterial phase image, well discernible from the surrounding liver tissue. MR images showed the tumor as low signal on T1WI, little high signal on T2WI （EPI） and marked high signal on DWI. DSA demonstrated the isolated tumor in the left lobe of the liver supplied by the enlarged arteries. Conclusion The rabbit model with VX2 liver tumor is suitable for interventional experimental study. The inject method is superior to the embed method. CT and MRI （T1WI/ T2WI（EPI）/DWI） have benefits to the image evaluation of tumor with their own superiority, especially the T2WI（EPI） and DWI. DSA can show the supplying arteries of the tumor clearly.

关 键 词：VX2 肝肿瘤 动物模型 体层摄影术 X线计算机 磁共振成像 数字减影血管造影 

分 类 号：R-332[医药卫生]