双自杀基因CD和TK对K562细胞体内外杀伤作用的实验研究  

Killing effect of double suicide genes mediated by lentivirus on K562 cells

作　　者：姜义荣[1] 万得胜[1] 陈万宁[1] 刘扶兴[1] 赖应昌[1] 陈小林[1] 祁妙华[1] 刘春生[2] 陈学良[2] 马道新[2] 

机构地区：[1]东莞市人民医院血液科,广东东莞523000 [2]山东大学齐鲁医院血液科,山东济南250031

出　　处：《中华肿瘤防治杂志》2006年第13期961-964,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金资助(30070321);东莞市科技计划项目资助(200417)

摘　　要：目的:探讨双自杀基因CD和TK对K562细胞的体内外抑制作用及前体药物对肿瘤的杀伤作用。方法:将目的基因转染入K562细胞,MTT法观察细胞在体内外的增殖状况及5-FC/GCV对转染细胞的杀伤作用,电子显微镜观察其超微结构变化。将K562/CDgly TK和K562细胞接种于裸鼠皮下,观察各种肿瘤细胞在体内的成瘤情况及对前体药物治疗的敏感性。结果:单独使用GCV或5-FC对K562及K562/CDgly TK细胞产生明显的杀伤作用,联合应用该2种药物对肿瘤细胞的杀伤作用更强。将K562细胞和K562/CDgly TK细胞接种于小鼠皮下后小鼠成瘤率为100%,GCV或5-FC可明显抑制裸鼠体内的肿瘤形成,联合应用GCV和5-FC治疗K562/CDglyTK细胞在小鼠体内形成的肿瘤,较单独应用GCV和5-FC及对照组小鼠形成的肿瘤体积明显缩小,生存期也明显延长。结论:双自杀基因在体内外对K562细胞均有明显的抑制作用,可增加前体药物GCV和5-FC对瘤细胞的杀伤率。OBJECTIVE： To study the killing effect of double suicide genes system on K562 cells in vivo and in vitro. METHODS： CDglyTK gene was transfected into K562 cells by using lipofectamine. K562 cells were infected with vi ral supernant. K562/CDgly TK cells were treated with 5-FC and/or GCV. The mice were divided into three groups randomly： tumor formation group, tumor inhibition group and tumor therapy group. Each mouse was implanted with K562/CDgly TK cells or K562 cells. RESULTS： The killing effect of 5-FC and GCV in combination on K562/CDglyTK was more effective than that of using 5-FC or GCV alone. In vivo study, the rate of tumor formation was 100%. Double suicide genes could suppress tumor formation of K562/CDglyTK cells. After the mice were treated with double suicide genes, the median tumor volume of mice implanted with K562/CDglyTK cells decreased obviously compared with the control group. Their median survival was significantly prolonged. CONCLUSIONS： Double suieide genes are more effective for killing effect on K562 cells in vivo and in vitro. It may be applicable for clinical gene therapy.

关 键 词：转基因 K562细胞 慢病毒属 双自杀基因 

分 类 号：R733.7[医药卫生—肿瘤]