广东鼻咽癌患者ATM基因127464G/A位点单核苷酸多态性研究  

Study on single nucleotide polymorphisms of ATM Gene 127464G/A site in Cantonese patients nasopharyngeal carcinoma

作　　者：王宏梅[1] 陈龙华[1] 李启生[1] 郑小康[1] 吴德华[1] 张洪波[1] 

机构地区：[1]南方医科大学南方医院放疗科,广东广州510515

出　　处：《中华肿瘤防治杂志》2006年第13期971-973,共3页Chinese Journal of Cancer Prevention and Treatment

基　　金：广东省科技计划项目资助(2004B30301011);广东省自然科学基金项目资助(2005004776)

摘　　要：目的:研究ATM基因单核苷酸多态性位点127464G/A与鼻咽癌(nasopharyngeal carcinoma,NPC)的发生关系。方法:应用非变性聚丙烯酰胺凝胶电泳结合测序方法对38例广东NPC患者及40例对照样本ATM基因127464G/A基因分型。结果:在NPC患者及对照个体中G/A杂合型频率分别为57.9%(22/38)、62.5%(25/40),G/G纯合型频率分别为13.2%(5/38)、17.5%(7/40),A/A纯合型频率分别为28.9%(11/38)、20%(8/40)。结论:体细胞ATM基因127464G/A位点变异在广东NPC患者与正常人群中分布差异无统计学意义,推测其与广东NPC的遗传易感性相关性不大。OBJECTIVE：To study the relationship between the single nucleotide ploymorphisms of Ataxia-telangiectasia mutated （ATM） gene 127464G/A site and the occurrence of Cantonese nasopharyngeal carcinoma （NPC）, METHODS： 127464G/A genotyping was processed by the methods of non-denaturing polyacrylamide gel electrophoresis and direct gene sequencing, and the blood sqmple were from 38 Cantonese nasopharyngeal carcinoma patients and 40 healthy individuals, RESULTS,. The heterozygote G/A genotype frequencies in NPC patients and healthy individuals were 57.89% （44/76） and 51.25% （41/80）, the homozygote G/G freqnencies were 13.2 %（5/38） and 17.5% （7/40）, and the homozygote A/A freqnencies were 28, 9% （11/38） and 20% （8/40）, respectively. CONCLUSIONS： With the statistics of Chi-square analysis, there are no significant differences between NPC patients and the control samples. The results indicate that somatic ATM gene 127464G/A variants have little relations with the genetic susceptibility in Cantonese nasopharyngeal carcinogenesis.

关 键 词：鼻咽肿瘤/遗传学 癌基因 多态性 单核苷酸 

分 类 号：R739.63[医药卫生—肿瘤]