制作角膜缘干细胞完全缺乏动物模型的新方法  

New method to creat rabbit model of limbus stem cell deficiency

作　　者：蔺琪[1] 吕岚[1] 金涛[1] 赵晓玉[1] 邱波[1] 

机构地区：[1]首都医科大学附属北京同仁医院眼科,北京市100730

出　　处：《眼科新进展》2006年第8期565-568,共4页Recent Advances in Ophthalmology

基　　金：国家高技术研究发展计划(863计划)基金资助(编号:2003AA205070-04)~~

摘　　要：目的提出一种新的角膜缘干细胞完全缺乏模型制作方法，为干细胞缺乏疾病的治疗性研究提供可靠的模型。方法利用9mm环钻划界，板层切除角膜组织，通过临床评分、印迹细胞学、组织病理及免疫组织化学验证模型的成功。结果板层切除术后30～45d，模型眼角膜混浊、上皮缺损、大量新生血管长入，印迹细胞学可见PAS（＋）的杯状细胞，组织病理学检查上皮符合结膜细胞表型，免疫荧光染色可见结膜杯状细胞特异性标志MUC5AC阳性表达。结论环钻划界加板层切除法为一种可靠、有效的角膜缘干细胞完全缺乏模型制作方法。Objective To explore a new method on creating total limbus stem cell deficient （LSCD） model,and provide the reliable model for the remedial study of stem cell deficiency. Methods L2mellar kerateetorny extending 1mm outside the limbus was made with a 9 mm trephine. Clinical evaluation, impression cytology（IC）, histology and immunofluorescenee staining were performed to vertify the establishment of total LSCD model. Results 30 - 45 days after the kerateetomy, the corneal opacity, neovascularization and epithelial defects appeared. Corneal IC indicated goblet cells; HE staining showed that eonJunetival phenotypie and monoclonal antibody （45M1） against MUCSAC were expressed. Conclusion Trepanation and lamellar kerateetorny is an effective and dependable method to ereat total LSCD model.

关 键 词：角膜缘干细胞 动物模型 印迹细胞学 

分 类 号：R771[医药卫生—眼科]